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3D Cell Culture Perfusion System - PerfusionPal™

3D Cell Culture Perfusion System - PerfusionPal™

Cambridge Bioscience offers a 3D cell culture perfusion system providing superior oxygenation to enhance cell function and viability. Extending cell survival from days to weeks, even for primary cells, PerfusionPal™ is the only system capable of continuous perfusion of 12 statistically independent organ tissue/cell models in their own media with a single tube and a single pump.

PerfusionPal Technology
PerfusionPal is an open insert system with two gas exchange surfaces. Air is exchanged at the top of the well and the blood substitute solution at the bottom of the culture. Drugs, reagents and media can be easily added and removed directly from each well using a micropipette. Precious developmental drugs and biologics will not be wasted or absorbed in the fluidics of the system leading to reliable and reproducible results in drug testing and translational research.

PerfusionPal


Benefits Of Using PerfusionPal
• Physiologically relevant interstitial perfusion
• Superior oxygenation
• Reliable & consistent
• Single tube & single pump
• Perfuse 12 individual culture wells
• Easily add or remove media, drugs & reagents
• Zero dead volume in fluidics

Applications
PerfusionPal's innovative technology concentrates the secretome, metabolome and signalling molecules produced by the cells in the well. These molecules may be concentrated enough to facilitate new therapeutic biomarker efficacy or therapy surveillance.
Applications include:
• Small molecule drug testing - ADME/Tox
• Biopharmaceutical testing - on target efficacy/ off-target toxicity of antibody-drug-conjugates & multi-specific antibodies
• Culture advanced tumour 3D models over long periods of time in vitro
• Model advanced, vascularised tumour models with heterogeneous cell populations, extracellular matrix, & intra-tumour supply of drugs to more closely mimic a patient’s tumour at the time of treatment

Phase I Drug Metabolism : Culturing human hepatocytes in the PerfusionPal™ consistently enhances the baseline activity of the key drug metabolising enzyme, CYP1A

Culturing human hepatocytes in the PerfusionPal consistently enhances the baseline activity of the key drug metabolizing enzyme, CYP1A

Prior to EROD assays, HepaRG™ cells, iPSC-derived Hepatocytes 2.0 (Cellular Dynamics) & a 10-donor pool primary hepatocytes (BioreclamationIVT Cryoplateable Liverpool)™ were maintained in culture for 5 days. HepG2 cells, that had significantly lower activity from all other cell types, were maintained for 7 days. The results were normalised to respective 2D controls for each cell type. 2D refers to 2D cell cultures, 3D refers to 3D cultures cultured in SeedEZ, 3D static refers to 3D cultures cultured in SeedEZ in unperfused PerfusionPal™ with the blood substitute (the effect of superior oxygenation), and 3D perfused refers to 3D cultures cultured in SeedEZ and perfused in PerfusionPal™. *p<0.05, **p<0.01, ***p<0.001

Cellular Metabolism: Increase in cellular respiratory metabolism in PerfusionPal™ for HepG2 liver cell line, HepaRG differentiated hepatobiliary cells & BT-474 HER2+ breast cancer cell line

Increase in cellular respiratory metabolism in PerfusionPal for HepG2 liver cell line, HepaRG differentiated hepatobiliary cells, and BT-474 HER2+ breast cancer cell line.

AlamarBlue assay. The results were normalised to respective 3D controls for each cell type. 3D refers to 3D cultures cultured in SeedEZ, 3D static refers to 3D cultures cultured in SeedEZ in unperfused PerfusionPal™ with the blood substitute (the effect of superior oxygenation), & 3D perfused refers to 3D cultures cultured in SeedEZ & perfused in PerfusionPal™. *p<0.05, **p<0.01, ***p<0.001

Long-Term Culture of Complex Culture Model

Primary Cell Brain Culture    Hepatobiliary Model

 

 

 

 

 

 

 

Breast Cancer Cell Culture

Primary brain tri-culture model & differentiated liver hepatobiliary model perfused in parallel in PerfusionPal™ for 4 weeks prior to imaging; and breast cancer model at 10X

Testimonial
“My lab is one of the first in the UK to use SeedEZ™ and PerfusionPal™. SeedEZ™ is simple to use, robust and easy to handle. We have used it to culture breast cancer cells and they grow very well. PerfusionPal is also great – simple to set up and unlike some platforms there are no leaks. Once it is set up you simply put it in the incubator and let the experiment run, safe in the knowledge that the system will take care of itself. There are lots of platforms on the market nowadays, but we find SeedEZ and PerfusionPal a simple and effective way of performing 3D cell culture, which works reliably and consistently”.

Professor Valerie Speirs
LICAP Breast Research Group, Leeds Institute of Cancer & Pathology, St James’ Hospital, Beckett Street, Leeds LS9 7TF

"We tried a number of culture systems to help us expand our 3D culture applications. PerfusionPal was just the solution we needed. It was very easy to set up and use. We look forward to using it in future studies."

Dan Close
Chief Scientific Officer
490 BioTech


PerfusionPal™ Citations

Material Available For Download
Perfusion Pal™ PP-A012 Technical Data Sheet

Products

Note: availability depends on country - see product detail page.

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Applications: Cell and tissue culture
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Applications: Cell and tissue culture
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Applications: Cell and tissue culture
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Applications: 3D cell migration, invasion and chemo-invasion assays
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Applications: 3D cell migration, invasion and chemo-invasion assays
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Applications: Cell and tissue culture
CAS Number: 306-91-2
Molecular Weight: 624
Structure: 1,1,2,2,3,3,4,4,4a,4b,5,5,6,6,7,7,8,8,8a,9,9,10,10
Compound Purity: 100%
View product detail
Applications: Cell and tissue culture
CAS Number: 306-91-2
Molecular Weight: 624
Structure: 1,1,2,2,3,3,4,4,4a,4b,5,5,6,6,7,7,8,8,8a,9,9,10,10
Compound Purity: 100%
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