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Microbiomic & Metagenomic Research Tools
Log Distribution Microbial Community Standard

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ZymoBIOMICS™ Log Distribution Microbial & DNA Standards

ZymoBIOMICS™ Log Distribution Microbial & DNA Standards

Cambridge Bioscience offers a well-defined, mock microbial community standard of 8 bacterial and 2 fungal strains mixed to create a log distributed abundance. With a range over 102 to 108 cells, ZymoBIOMICS™ microbial community standard II can be used to assess the detection limit of a microbiomics workflow and may also be used as a positive control for routine sequencing.

In addition, Cambridge Bioscience also offers an accurately characterised mixture of genomic DNA of 8 bacterial and 2 fungal strains constructed by pooling DNA extracted from pure cultures of the ten strains. The ZymoBIOMICS™ microbial community DNA standard II log distribution enables a detection limit of as low as the DNA of three microbes.

Benefits Of Using These Log Distribution Standards
• Well defined & characterised
• < 0.01% impurities
• Assess the accuracy of microbiome measurements
• Ideal for QC of microbiome measurements

ZymoBIOMICS Microbial Community Standard II (Log Distribution)

The microbial composition of the standard measured by NGS shotgun sequencing as compared to the defined composition. After mixing, the microbial composition of the standard was confirmed using deep Illumina® shotgun sequencing. Briefly, the genomic DNA was extracted using the ZymoBIOMICS™ DNA Miniprep. Library preparation was performed using an in-house protocol. Shotgun sequencing was performed using Illumina HiSeq™ or MiSeq™. Microbial abundance was estimated based on the number of reads that were mapped to references genomes of the organisms.

Microbial Composition

ZymoBIOMICS Microbial Community Log Distribution Composition

1 The theoretical composition in terms of 16S (or 16S & 18S) rRNA gene abundance was calculated from theoretical genomic DNA composition with the following formula: 16S/18S copy number = total genomic DNA (g) × unit conversion constant (bp/g) / genome size (bp) × 16S/18S copy number per genome. Use this as reference when performing 16S targeted sequencing.
2 The theoretical composition in terms of genome copy number was calculated from theoretical genomic DNA composition with the following formula: genome copy number = total genomic DNA (g) × unit conversion constant (bp/g) / genome size (bp). Use this as reference when inferring microbial abundance from shotgun sequencing data based on read depth.
3 The theoretical composition in terms of cell number was calculated from theoretical genomic DNA composition with the following formula: cell number = total genomic DNA (g) × unit conversion constant (bp/g) / genome size (bp)/ploidy.

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Material Available For Download
ZymoBIOMICS™ Microbial Community Standard II (Log Distribution) Protocol
ZymoBIOMICS™ Microbial Community Standard II (Log Distribution) SDS
ZymoBIOMICS™Microbial Community DNA Standard II (Log Distribution) Protocol
ZymoBIOMICS™ Microbial Community DNA Standard II (Log Distribution) SDS

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