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Viromer® Plasmid

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Nucleic Acid Labelling Kit - Label IT®-MFP488

Nucleic Acid Labelling Kit - Label IT®-MFP488

Cambridge Bioscience offers a non-enzymatic chemical labelling kit that facilitates the direct covalent attachment of a highly photostable MFP488 label to DNA or RNA template in a single step. Optimised for a wide range of molecular biology applications including gene expression, gene knock-down and nucleic acid probe hybridisation, the Label IT®-MFP488 kit offer researchers a more robust alternative to fluorescein.

Benefits Of Using The Label IT®-MFP488 Kit
• High photostability
• Spectrally similar to fluorescein
• Stable at low pH
• Label any DNA or RNA template
• Single-step chemical method
• Adjustable labelling density
• Covalent mechanism does not impact hybridisation performance

Label IT® MFP488 Labeled Plasmid DNA is More Resistant to the Effects of Low pH as Compared to Label IT® Fluorescein Labeled Plasmid DNA.

Label IT® MFP488 Labelled Plasmid DNA is More Resistant to the Effects of Low pH as Compared to Label IT® Fluorescein Labelled Plasmid DNA. Plasmid DNA was labelled at a low density, 1 label per 65 bp, using Label IT® MFP488 or Label IT® Fluorescein Nucleic Acid Labelling Kits. Purified DNA was subsequently diluted in various pH buffers (50 mM HEPES, pH 8, 100 mM MES, pH 5.4, and 100 mM MES, pH 3). Fluorescence was measured using a Tecan Genios Fluorometer and normalised to the respective control measured at 50 mM HEPES, pH 8.

Label IT® MFP488 Labeled Plasmid DNA is Less Sensitive to Photobleaching as Compared to Label IT® Fluorescein Labeled Plasmid DNA.

Label IT® MFP488 Labeled Plasmid DNA is Less Sensitive to Photobleaching as Compared to Label IT® Fluorescein Labeled Plasmid DNA. (A) Plasmid DNA was labelled with Label IT® MFP488 or Label IT® Fluorescein.  Purified DNA was transfected into HeLa cells using the TransIT®-LT1 Transfection Reagent at a 3:1 reagent-to-DNA ratio (vol:wt). Two hours post transfection labelled DNA was visualised using a 63X oil immersion objective on a Zeiss Axiovert Inverted Fluorescence microscope. Images were captured before & after a 2 minute photobleaching period from a FITC light source. (B) The ratio (X-axis) of the brightness of individual puncta was calculated by dividing the photobleached value for fluorescence brightness by the value obtained from non-photobleached values for four separate images using Image J software (NIH), the average and standard deviation are shown. Photobleaching was also measured after 5 minutes for Label IT-MFP488 labeled plasmid DNA & the signal was still 4-fold higher than Label IT® Fluorescein labeled plasmid DNA at 2 minutes (data not shown).

Material Available For Download
Label IT® Nucleic Acid Labelling Kits Protocol
Label IT® Nucleic Acid Labelling Kits Quick Reference Protocol
LabelIT® Nucleic Acid Labelling Kits SDS

Nucleic Acid Labelling Kits - Label IT®
All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra. To see the full range of Label IT® kits available, Click Here.

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