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High Efficiency Competent Cells for Protein Expression

Detergents for Membrane Protein Purification

EvaGreen®

Transfection Ready Plasmid DNA In 20 Minutes With ZymoPURE II

Thermal shift protein stability kit – GloMelt™

Thermal shift protein stability kit – GloMelt™

The GloMelt™ kit from Biotium can be used to detect protein unfolding or measure thermal stability by generating fluorescence enhancement upon dye binding to hydrophobic regions of denatured proteins. Optimised for detection in the SYBR® Green channel of qPCR instruments, the GloMelt™ dye generates a strong signal, allowing for use with low reaction volumes and low protein concentrations.

Benefits of using the GloMelt™ kits
•    Strong signal
•    Compatible with low reaction volumes and low protein concentrations
•    Results obtained in 30 minutes
•    Requires less protein than circular dichroism and differential scanning calorimetry
•    Compatible with high concentrations of protein stabilisers and destabilisers
•    Performs very well in high detergent concentrations
•    Compatible with high throughput screening
•    Compatible with ROX dye to increase replicate consistency
•    Cost-effective

Applications
•    Optimise buffer formulation for protein stability and storage
•    Determine how mutations affect protein stability
•    Rapidly screen small molecule drug candidates and other ligands for protein binding

The environmentally sensitive fluorescent dye can be used to monitor the temperature-dependent unfolding of a protein

The environmentally sensitive fluorescent dye can be used to monitor the temperature-dependent unfolding of a protein

The protein’s melting temperature (Tm) is a reporter of the protein’s thermal stability.

IgG melt curve plots in the presence of detergent: GloMelt™ and SYPRO® Orange

IgG melt curve plots in the presence of detergent: GloMelt™ and SYPRO® Orange

A thermal shift assay was performed on 20 µg IgG in the presence of 5X SYPRO® Orange or 1X GloMelt™ dye, using a QuantStudio™ 5 qPCR system. The presence of detergent inhibited the SYPRO® Orange assay, but did not significantly affect the GloMelt™ curve.

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