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Product types:
High Efficiency Competent Cells for Protein Expression
Electrocompetent Cells With The Highest Transformation Efficiency

Low Toxicity Live Cell Nuclear Stains

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Vmax™ Express Chemically Competent Cells

Vmax™ Express Chemically Competent Cells

Cambridge Bioscience offers a novel, fast-growing bacterial strain for high-level recombinant protein expression. Producing twice as much biomass and quadruple the amount of protein faster than E. coli, Vmax™ express chemically competent cells offer increased compatibility with a range of proteins that may be difficult to express in E.coli, supported by robust transcription and translation systems.

Vmax™ express chemically competent cells can be cultured using routine growth medium, as well as commercial auto-induction media, or Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass).

Benefits Of Using Vmax™ Express Chemically Competent Cells
• More than double the amount of protein/ml than E. coli
• Two-fold improvement in replication time
• Increased compatibility with proteins which are difficult to express in E. coli
• Protein purification in only 3 days

Vmax™ Express - Fastest Growth Rate & Generates Greater Biomass
Growth comparison of Vmax™ with other E. coli strains. Vmax™ express demonstrates faster growth and a greater accumulation of biomass as compared to other common laboratory strains. Panel A. Overnight cultures of the indicated strains grown in Brain Heart Infusion Broth + v2 salts were inoculated to an OD600 of 0.03 and grown at the E. coli optimum of 37°C. At the indicated times, the OD600 of each culture was determined. Panel B. LB-Miller plates agar plates were streaked with the indicated strains and incubated at 37°C. Images of the growth were recorded at the times indicated.

Vmax™ Express Generates Larger Amounts of Soluble Protein

Expression of Green Fluorescent Protein (GFP) in Vmax™. To evaluate expression levels in Vmax™ Express, a plasmid expressing Green Fluorescent Protein (GFP) was introduced to cells and induced with IPTG at an OD of 0.5. Except where indicated in the figure, expression was performed at 30°C. At the time points indicated, samples of IPTG induced (I) and uninduced (U) were taken for analysis. Panel A. UV light was used to visualise active GFP in equal volumes of each cell lysate. In Vmax™ express cells, detectable signal was observed in all induced samples. Panel B. Lysates from panel A were examined by SDS-PAGE followed by Coomassie Blue staining to visualise the amounts of expressed 27 kDa GFP protein.

• Lab scale expression of recombinant proteins
• Large scale expression of recombinant proteins
• Expression of proteins that express at low levels or as inclusion bodies in E. coli
• Expression of active proteins

Material Available To Download
Vmax™ Express Chemically Competent Cells User Manual


Note: availability depends on country - see product detail page.

Contact Our SGI-DNA Specialist Emma
SGi-DNA Vmax™ Express Chemically Competent and Electrocompetent Cells