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DNA Fragment Library Preparation - NxSeq® UltraLow DNA Library Kit

DNA Fragment Library Preparation - NxSeq® UltraLow DNA Library Kit

Cambridge Bioscience offers a low-input, high efficiency DNA fragment library preparation kit compatible with Illumina platforms. Containing the buffers and enzymes needed to make low-bias, PCR-amplified DNA fragment libraries for whole genome sequencing, the NxSeq® UltraLow DNA Library Kit is compatible with as little as 50 pg up to 75 ng of sheared/fragmented genomic DNA from all sample types including human and bacteria.

Benefits Of Using The NxSeq® UltraLow DNA Library Kit
• Use as little as 50 pg up to 75 ng of sheared/fragmented DNA
• Compatible with Illumina sequencers
• 3 hour protocol
• High efficiency adaptor ligation
• Produces high quality complex libraries
• Minimal bias
• Cost effective

Multiple Library Prep Workflow & Timing Comparison

Comparison Of Multiple Library Prep Workflows & Timing

Comparison Of Multiple Library Prep Workflows & Timing

The workflows illustrated correspond to the following kits: Lucigen, NxSeq® UltraLow DNA Library Kit; Illumina, TruSeq Nano DNA LT Library Preparation Kit; Kapa, Hyper Prep Kit with KAPA Library Amplification Primer Mix; NEB, NEBNext Ultra II DNA Library Prep Kit for Illumina.

Applications
• de novo whole genome sequencing
• Resequencing
• Exome-seq
• ChIP-seq
• FFPE DNA samples

Formats Available
NxSeq® UltraLow DNA Library Kit 12 Reactions
A NxSeq Single Indexing Kit is required to complete library prep with the NxSeq® UltraLow DNA Library Kit, 12 Reactions and must be purchased separately:
• NxSeq® Single Indexing Kit, Set A (Catalogue number 15100-01)
• NxSeq® Single Indexing Kit, Set B (Catalogue number 15200-1)

NxSeq® UltraLow DNA Library Kit 96 Reactions
Designed for customers building Illumina-compatible DNA fragment libraries in 96-well plates, the NxSeq® HT Dual Indexing Kit is necessary for use with the NxSeq® UltraLow DNA Library Kit, 96 Reactions. The NxSeq® HT Dual Indexing Kit (catalogue number 15300-1):
• contains sufficient reagents to enable library prep on automated systems
• includes 2 coloured primers to check correct plate dispensation

NxSeq® AmpFREE Low DNA Library Kit
We also offer the NxSeq® AmpFREE Low DNA Library Kit allowing you to use low input amounts without the need for PCR amplification prior to sequencing. For more information, please Click Here.

Material Available For Download
NxSeq® UltraLow DNA Library Kit, 12 Reactions Protocol
NxSeq® UltraLow DNA Library Kit, 96 Reactions Protocol
NxSeq® UltraLow DNA Library Kit, 12 Reactions & NxSeq® Single Indexing Kits, Sets A and B FAQs

Products

Note: availability depends on country - see product detail page.

Contact Our Lucigen Specialist, Doaa