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Enhance Taq DNA Polymerase During Annealing & Elongation - ThermaGo™

Enhance Taq DNA Polymerase During Annealing & Elongation - ThermaGo™

Cambridge Bioscience offers a reagent to suppress mis-priming during PCR annealing and extension, preventing scatter due to unwanted chimera and mis-priming product formation.  A double-stranded, chemically modified nucleic acid, ThermaGo™ enhances the specificity of Taq DNA polymerase at elevated temperatures during PCR annealing and elongation, increasing the reproducibility of both real-time and end-point analyses.

ThermaGo™  works synergistically with ThermaStop™ to provide improved PCR performance before, during and after amplification. To find out more about ThermaStop™ please Click Here.

Benefits Of Using ThermaGo™
• Improves reproducibility of technical & biological replicates
• Increases detection of low-copy number targets by suppressing non-specific products
• Reduces signal scatter in standard & digital PCR
• Reduces mis-priming & chimera production prior to NGS
• Improves end-point genotyping

ThermaGo™  reduces scatter, increases product signals & allows greater separation of signals from homozygous & heterozygous genomes during real-time or end-point analyses. Lines represent probe signals from a Taqman probe against allele A: red lines - homozygous AA target, blue lines - heterozygote Aa target, green lines - homozygous aa target.

Usage
• 1 unit of ThermaGo™  = amount required for maximum improvements in specificity, yield & reproducibility in amplification reactions containing 1 unit of Taq DNA polymerase in 25 μl
• Add an equal number of units of ThermaGo™ and Taq DNA polymerase to a PCR master mix
• To further optimise, test ThermaGo™ in range of 0.5 to ≥2.0 units per unit of Taq DNA polymerase
• NB: very high levels of ThermaGo™ can inhibit amplification thereby reducing product yield

ThermaGo™ works synergistically with ThermaStop™ to provide improved PCR performance before, during and after amplification. To find out more about ThermaStop™ please Click Here.

Material Available For Download
ThermaGo™ Usage Instructions
ThermaGo™ Product Bulletin
 

Products

Note: availability depends on country - see product detail page.

View product detail
Applications: PCR, qPCR,Multiplex PCR
View product detail
Applications: PCR, qPCR,Multiplex PCR