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Electrocompetent Cells With The Highest Transformation Efficiency

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Vmax™ Express Electrocompetent Cells

Vmax™ Express Electrocompetent Cells

Cambridge Bioscience offers a novel, fast-growing bacterial strain for high-level recombinant protein expression. Producing twice as much biomass and quadruple the amount of protein faster than E. coli, Vmax™ express electrocompetent cells offer increased compatibility with a range of proteins that may be difficult to express in E.coli, supported by robust transcription and translation systems.

Vmax™ express electrocompetent cells can be cultured using routine growth medium, as well as commercial auto-induction media, or Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass).

Benefits Of Using Vmax™ Express Electrocompetent Cells
• More than double the amount of protein/ml than E. coli
• Two-fold improvement in replication time
• Increased compatibility with proteins difficult to express in E. coli
• Protein purification in only 3 days



Vmax™ Express - Fastest Growth Rate & Generates Greater Biomass
    
Growth comparison of Vmax™ with other E. coli strains. Vmax™ express demonstrates faster growth and a greater accumulation of biomass as compared to other common laboratory strains. Panel A. Overnight cultures of the indicated strains grown in Brain Heart Infusion Broth + v2 salts were inoculated to an OD600 of 0.03 and grown at the E. coli optimum of 37°C. At the indicated times, the OD600 of each culture was determined. Panel B. LB-Miller plates agar plates were streaked with the indicated strains and incubated at 37°C. Images of the growth were recorded at the times indicated.

Vmax™ Express Generates Larger Amounts of Soluble Protein


Expression of Green Fluorescent Protein (GFP) in Vmax. To evaluate expression levels in Vmax™ express, a plasmid expressing Green Fluorescent Protein (GFP) was introduced to cells and induced with IPTG at an OD of 0.5. Except where indicated in the figure, expression was performed at 30°C. At the time points indicated, samples of IPTG induced (I) and uninduced (U) were taken for analysis. Panel A. UV light was used to visualise active GFP in equal volumes of each cell lysate. In Vmax™ express cells, detectable signal was observed in all induced samples. Panel B. Lysates from panel A were examined by SDS-PAGE followed by Coomassie Blue staining to visualise the amounts of expressed 27 kDa GFP protein.

Applications
• Lab scale expression of recombinant proteins
• Large scale expression of recombinant proteins
• Expression of proteins that express at low levels or as inclusion bodies in E. coli
• Expression of active proteins

Material Available To Download
Vmax™ Express Electrocompetent Cells User Manual

Products

Note: availability depends on country - see product detail page.

SGi-DNA Vmax™ Express Chemically Competent and Electrocompetent Cells
Tonbo Biosciences