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Total RNA Purification From Acid-Guanidinium-Phenol Reagents
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DNA/RNA Shield™ swab collection tubes

DNA/RNA Shield™ swab collection tubes
DNA/RNA Shield™ simple swab sample collection devices enable ambient temperature nucleic preservation and pathogen inactivation with no need for reagent removal prior to purification. Samples collected where a swab may be deemed appropriate are stabilised in DNA/RNA Shield™ into a collection device or a collection tube ready for sample homogenisation.

Benefits of using the DNA/RNA Shield™ swab collection tubes
• Compatible with any sample appropriate for swab collection
• Pathogen inactivation
• RNA stable at room temperature for one month
• DNA stable at room temperature for two years
• Ready for nucleic-acid extraction
 

Product specifications
1. DNA/RNA Shield™ collection tube w/ swab

• 12 x 80 mm screwcap vial prefilled with DNA/RNA Shield™ (1 mL or 2 mL)
• Nylon swab with 80mm breakpoint
2. DNA/RNA Shield™ lysis tube w/ swab (microbe)
• 2 mL screwcap tube prefilled with DNA/RNA Shield™ (1 mL)
• Tube prefilled with ultra-high density BashingBeads™ (0.5 mm & 0.1 mm beads) for homogenisation
• Nylon swab with 20mm breakpoint

Saliva stabilisation in DNA/RNA Shield™
 
 
Nucleic acid in saliva is effectively stabilised in DNA/RNA Shield™ at ambient temperature. Graphs show: spike-in DNA and RNA controls from saliva purified at the indicated time points and analysed by (RT)qPCR. Controls: HSV-1 and HIV (AcroMetrix, Life Technologies).

Viruses, bacteria and yeast effectively inactivated by DNA/RNA Shield™
Samples containing the infectious agent (virus, bacteria, yeast) were treated with DNA/RNA Shield™ or mock (PBS) treated for five minutes. Titer (PFU) was subsequently determined by plaque assay. Validated by: Influenza A - D. Poole and Prof. A. Mehle, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison; Ebola (Kikwit) - L. Avena and Dr. A. Griffiths, Department of Virology and Immunology, Texas Biomedical Research Institute; HSV-1/2 - H. Oh, F. Diaz and Prof. D. Knipe, Virology Program, Harvard Medical School; E. coli, L. fermentum, B. subtilis, S. cerevisiae– Zymo Research Corporation)
*Disclaimer: This graph only displays results from E. coliinactivation. Each microbe was tested independently and were combined into one graph for brevity. Bacterial cultures were grown between 108 - 109 cells and yest cultures were grown between 107 - 108 cells.

Streamlined purification from samples in DNA/RNA Shield™
High-quality DNA and RNA is efffectively purified from blood stored in DNA/RNA Shield™ using the Quick-DNA™ and Quick-RNA™ kits from Zymo Research respectively. High molecular weight DNA was intact with no apparent degradation. Also, RNA was high quality, DNA-free and incuded small RNAs when purified from DNA/RNA Shield™. This was not the case using another supplier’s product (data not shown).

Microbiomics portfolio
Our ZymoBIOMICS™ portfolio offers a complete workflow from streamlined collection through to analysis. Find out more Here

Material available for download
DNA/RNA Shield™ Collection Tube w/ Swab Protocol

DNA/RNA Shield™References

 

Products

Note: availability depends on country - see product detail page.

Speak To Our Zymo Research Specialist Emma