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Transfection-Ready Plasmid DNA in <20 Minutes - ZymopPURE II Midi & Maxiprep Kits
Request A Free ZymoPURE II™ Sample

DNA Methylation Library Prep Kit

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Ultra-Pure Plasmid DNA In 20 Minutes – ZymoPURE II™ Midiprep & Maxiprep

Ultra-Pure Plasmid DNA In 20 Minutes – ZymoPURE II™ Midiprep & Maxiprep

Cambridge Bioscience offers the ZymoPURE II™ midiprep and maxiprep kits for the isolation of up to 400 µg (midiprep) or 1.2 mg (maxiprep) of salt, protein and RNA-free vaccine/transfection quality plasmid DNA from E. coli in less than 20 minutes.

Benefits of Using the ZymoPURE II™ Midi & Maxiprep  Kits
• Ultra-pure vaccine/transfection grade DNA produced
• 20 minute protocol
• Up to 400 µg (midiprep)/1.2 mg (maxiprep) plasmid DNA isolated
• Endotoxin levels of ≤ 0.025 EU/µg Plasmid DNA (if using additional EndoZero™ columns provided)
• Simple protocol
• Reliable, consistent performance
• DNA ready for immediate use
• Wide downstream application compatibility
• Free samples available to trial

ZymoPURE II™ Midiprep & Maxiprep Protocol

Ultra-Pure Plasmid DNA In 20 Minutes – ZymoPURE II™


ZymoPURE II™ Plasmid DNA Maxiprep Kit

  ZymoPURE II™ Maxiprep Kit Supplier Q Endofree Maxiprep Supplier Q Maxiprep
Maximum Yield 1200 µg 500 µg 500 µg
Endotoxins ≤ 0.025 EU/µg ≤ 0.1 EU/µg ≤ 10 EU/µg
Time per prep < 20 min 150 min 160 min
Hands-on time 15 min 45 min 45 min
Loads via vacuum or centrifuge (no gravity flow)  Yes No No
Elute from a spin column (no precipitation) Yes No No
Vaccine grade* Yes No No
In vivo & gene therapy grade Yes Yes No
Transfection grade (sensitive & primary cells) Yes Yes No

* Endotoxins below FDA limit for vaccines <0.04 EU/µg of plasmid DNA

Midiprep Testimonials
“With your kit, I got twice the amount of DNA that I usually get with Invitrogen's midi kit, in half the time. Can't beat that!”
D. G., Immunology Consultants Laboratory

“I have used the Promega and Qiagen kits, but even with following all the steps carefully, I have never got such high quality and concentrated DNA.”
P. J., University of Miami

Maxiprep Testimonials
“We were impressed by the high yield of plasmid DNA from this kit! From a 150 ml culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”
C. S., Abbott Laboratories

“DNA obtained from using the ZymoPURE kit was ten times the amount of DNA obtained from using the QIAGEN kit.”
J. M., Vanderbilt University

Free Samples
Request a free ZymoPURE II™ Midiprep sample Here
Request a free ZymoPURE II™ Maxiprep sample Here

Material Available To Download
ZymoPURE II™ Plasmid Midiprep Protocol
ZymoPURE II™ Plasmid Maxiprep Protocol

Lipofectamine is a registered trademark of Life Technologies Corporation. ONE-Glo™ is a trademark of Promega Corporation.


Note: availability depends on country - see product detail page.

Speak To Our Zymo Research Specialist Emma
Tonbo Biosciences