RNA extraction from FFPE samples - RNAstorm™

The RNAstorm™ RNA extraction kit is specifically for use with formalin-fixed (paraffin embedded or in fixative) tissue samples. Using proprietary CAT5™ technology, RNAstorm™ enhances the removal of formaldehyde-induced damage (including on alkylated and crosslinked bases) providing up to 1.5 µg high-quality, amplifiable RNA suitable for RNAseq, PCR, qPCR/RT-PCR or microarrays with only 50 minutes of hands-on time. This technology was developed by Cell Data Sciences researchers, building on research initiated at Stanford University and published in Nature Chemistry in 2015.

Benefits of using RNAstorm™
• Designed for use with formalin-fixed samples (paraffin embedded or in fixative)
• Up to 1.5 µg RNA isolated
• 50 minutes hands-on time
• All necessary reagents provided in spin-column format
• Total RNA (including miRNA) isolated
• CAT5™ technology enhances the removal of formaldehyde-induced damage
• Better template quality for reverse transcription and other enzymatic manipulation

Higher yields of amplifiable RNA
Significant improvements in RNA yield and quality (as measured by amount of amplifiable RNA) are seen using a prototype of the RNAstorm™ kit on FFPE samples from various tissues and ranging in age from 1976 to 2015.

Comparison of RNA recovery by quantitative RT-PCR from FFPE tissues. “Q” represents competitive commercial FFPE extraction kits.

Higher integrity RNA
Increased RNA integrity is observed for RNA extracted using the RNAstorm™ kit. An exemplary comparison with a popular commercial kit is shown below (Agilent Bioanalyzer RNA 6000 nano)