Sensitive, Antibody-Based Exosome Quantification - ExoELISA™

Cambridge Bioscience offers sensitive ELISA kits for the detection and quantification of specific exosomal subpopulations. Compatible with exosomes isolated by ExoQuick™, ExoQuick-TC™ or by ultracentrifugation techniques, the ExoELISA™ range enables the easy quantification of the 3 well-characterised exosome markers CD9, CD63 or CD81.

Quantification of Exosomes Using ExoELISA™

The exosome particles & their proteins are directly immobilised onto the wells of the microtiter plate. After binding, wells are coated with a block agent to prevent nonspecific binding of the detection antibody. The detection antibody is added to the wells for binding to specific antigen (e.g. CD63) protein on the exosomes. Extra-sensitive TMB is used for the assay read-out. ExoELISA kits come with exosome standards to make calibration curves.

Material Available for Download
Exosome Antibodies, Arrays and ELISAs Product Sheet
Exosome Antibody and ELISA Kits User Manual