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Culture, Quantify, Isolate & Amplify Exosomes
Breast Cancer Cell Optimisesd Transfection Reagent

Transfection Ready Plasmid DNA In 20 Minutes With ZymoPURE II

High efficiency exosome transfection system - EV Shuttle Kits

High efficiency exosome transfection system - EV Shuttle Kits
The EV Shuttle Kits provide an easy to use, exosome-based system for the delivery of nucleic acids including siRNA, miRNAs, mRNAs and plasmid DNA to a variety of hard-to-transfect cells. Taking advantage of the natural ability of exosomes to be internalised into cells, ready-to-use exosomes derived from human embryonic kidney cells (HEK 293) or murine dendritic cells (JAWS) can be transfected with your nucleic acid of choice, ready for transport and delivery to target cells.

Benefits of the EV Shuttle Kits
• High transfection efficiency
• Fast procedure
• Simple loading protocol
• Nonviral delivery platform
• Positive control included
• Stable cell line generation
• Boost exosome delivery with EV-Entry

EV-Entry System
The EV-Entry system enhances the rate of uptake of extracellular vesicles (EVs) by the recipient cells and the offloading of the transported cargo into the cytoplasm of the recipient cells, boosting the delivery of both RNA and protein cargo. The EV-Entry exosome delivery reagent is available separately (cat# EVEN105A-1/EVEN110A-1).

Exosome loading protocol
 
Simply combine the isolated exosomes provided in the kit with Exo-Fect solution and the nucleic acid of your choice to generate exosome delivery vehicles. The protocol takes less than an hour and is highly efficient at loading nucleic acids into exosomes for transport and delivery.

EV Shuttles and Lipofectamine® transfection comparison
 
Human EV shuttles were transfected with Exo-Fect solution and compared to Lipofectamine® 2000 on RAWS 264.7 cells using the exact same amount of Texas-Red labeled siRNA (100 pmol, 10x mag). EV shuttles are more efficient at transfecting siRNA into transfection-resistant cells in culture.

Material available for download
EV Shuttle Kits Product information sheet
Human EV Shuttle protocol
Mouse EV Shuttle protocol
EV-Entry System protocol

Products

Note: availability depends on country - see product detail page.

Contact our SBI Specialist, Doaa