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Highly Specific Fluorescence Assays
Culture, Quantify, Isolate & Amplify Exosomes
Active Endosome Escape Based Transfection Reagent

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Exosome Targeting Technology - XStamp™

Exosome Targeting Technology - XStamp™

Cambridge Bioscience offers a patented exosome surface display technology which can be used to place cellular "addresses" on exosomes in order to send them to specific destinations for cargo delivery. Based on a C-terminal fusion of the C1C2 domain from MFG-E8, protein sequences fused to the XStamp™ tag will display the protein ligand fusion on the surfaces of secreted exosomes enabling specific delivery.

Benefits of Using XStamp™ Exosome Targeting Technology
• Protein of choice displayed on secreted exosome surface
• Target specific cellular destinations
• Create stable cell lines producing XStamp™ exosomes

XStamp™ System

The protein ligand chosen to display on exosomes is cloned into the MCS & fused to the C1C2 domain. The XStamp™ lentivector also features a downstream EF1-Puromycin cassette for selection & stable cell line development.

MFG-E8 Localises to Exosome Surfaces (C1C2 domain)


Fluorescently-labelled antibodies for MFG-E8, CD58 & CD81 were used in combination for FACs analysis. CD58 is a known cell surface marker that is absent on exosomes. CD81 is known to be present both in cells & exosomes. The FACs data show that MFG-E8 is exclusively detected on exosomes & not present in the cells. 

NCAM-XStamp™ & Brain Homing Peptide 1 for Neural Targeting


NCAM-XStamp construct (cat no. XSTP721PA-1) & BHP1-XStamp construct (cat no. XSTP722PA-1) were transfected separately into mouse MSCs with SBI's XPack-GFP construct (cat no. XPAK530PA-1) for fluorescent tracking. After 48 h, the exosomes were collected. Equal amounts (100 ug) of control (No XStamp), NCAM-XStamp or BHP1-XStamp MSC exosomes were added to Neuro2a neuroblastoma cells in culture. Neurons were imaged for phase & GFP signals after 24 h incubation with the various exosomes to monitor GFP delivery mediated by the XStamp ligand coats.

Material Available for Download
XStamp™ Exosome Targeting Technology Datasheet
XStamp™ Exosome Targeting Technology Manual

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