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Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Pre-Adsorption Assay & Positive Control Antigens

Pre-Adsorption Assay & Positive Control Antigens
Cambridge Bioscience offers PrEST Antigens from Atlas Antibodies, for use as blocking agents and positive assay controls together with the corresponding antibody. Used as immunogens for the generation of Atlas Antibodies Triple A Polyclonals and PrecisA Monoclonals, the protein fragments consist of 50-150 amino acids and are designed to have as low sequence identity as possible to other human proteins. PrEST antigens are validated using ESI mass spectrometry, SDS page and the Nanodrop™ system.

Benefits Of Using PrEST Antigens
• Complementary to antibodies
• High specificity
• Well Validated

PrEST Antigens Applications
PrEST antigens are ideal for use in the following applications: 
1. Pre-adsorption assays for immunohistochemistry
2. Pre-adsorption assays for Western Blot
3. Positive controls in Western Blot

1. Pre-adsorption Assays For Immunohistochemistry
The examples below demonstrate IHC staining results following pre-adsorption of antibody with the corresponding PrEST Antigen. 
 
Anti-PLA2R1, AMAb90772 staining of human kidney, with and without preadsoption with PLA2R1 PrEST antigen
 







Anti-SOX11, HPA000536 staining of human mantle cell lymphoma, with and without preadsoption with SOX11 PrEST antigen
   






2. Pre-Adsorption Assays For Western Blot

Anti-STAT3, HPA001671 Western Blot using Anti-STAT3 antibody in human cell line RT-4, with and without pre-adsorption with STAT3 PrEST Antigen. 
 













3. Positive Controls In Western Blot
Anti-ALDH1A2, HPA010022 Western Blot using amounts of ALDH1A2 PrEST Antigen as loading control ranging from 0.5 to 50 nano grams. 
 












NanoDrop is a trademark of Thermo Fisher Scientific

Products

Note: availability depends on country - see product detail page.

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Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
View product detail
Applications: Blocking agent and positive assay control using corresponding antibodies.
Contact Our Atlas Antibodies Specialist, Hiten