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Electrocompetent Cells With The Highest Transformation Efficiency
Competent Cells Eliminating Endotoxin At Source
Enzyme-free, Directional Cloning & Expression Systems

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Chemically Competent Cells For Lac Based Protein Expression

Chemically Competent Cells For Lac Based Protein Expression

Cambridge Bioscience offers competent cells for tightly regulated control of protein expression whilst maintaining high yield in systems using the lac operator. Combining exceptional efficiency and a simple, reliable heat-shock procedure for chemical transformation, the HI-Control™ competent cells provide high protein yields with reduced leaky expression from small amounts of plasmid DNA.

Benefits of Using the HI-Control™ Competent Cells
• High protein yields - HI-Control™ 10G ≥1×109 cfu/µg & HI-Control™ BL21(DE3) ≥1×107 cfu/µg
• High efficiency - excellent transformation from small amounts of DNA
• Simple, reliable procedure
• Tightly regulated protein expression
• Reduced leaky expression
• Compatible with T7-lac and other lac based promoters
• Complete kit

Genotypes:
HI-Control™ BL21 (DE3)
F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)
HI-Control™ 10G
mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)

Material Available for Download
HI-Control® BL21(DE3) & HI-Control® 10G Chemically Competent Cells Manual

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