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Enzyme-free, Directional Cloning & Expression Systems

Casy - Rapid & Accurate Analysis Of Cellular Samples

Culture, Quantify, Isolate & Amplify Exosomes

Transfection Ready Plasmid DNA In 20 Minutes With ZymoPURE II

Exceptional yield exosome purification kit - Exo-spin™

Exceptional yield exosome purification kit - Exo-spin™

The Exo-spin™ exosome purification kit from Cell Guidance Systems purifies functional, whole exosomes (30-400 nm size) with superior yields and higher quality than many other alternative technologies. Based on a unique combination of precipitation and size exclusion chromatography purification with no organic buffers and requiring no ultracentrifugation, the Exo-spin™ kits are compatible with a variety of biological fluids including cell culture media, urine and saliva. The Exo-spin™ blood kits are suitable for use with blood, plasma and serum.

Benefits of using the Exo-spin™ kits
• Isolate intact whole exosomes
• Excellent yield and high levels of purity
• No ultracentrifugation required
• Simple and reliable
• Ultra-low protein and rRNA contamination
• No organic buffers
• Fast one hour protocol
• Wide sample compatibility

Cell culture medium, saliva, urine, human breast milk*, cerebrospinal fluid*
& any low protein biological fluids (1 x 109 particles/ml)
Sample starting volume
(per Exo-spin column)
Isolation method
Exo-spin™ kit
<1 ml to 50 ml Precipitation + SEC (centrifugation)
EX01
<1 ml to 75 ml Precipitation + SEC (fractionation by gravity flow)
EX05
75 ml to 500 ml Precipitation + SEC (fractionation by gravity flow)
EX04
Blood samples (plasma and sera)†
SERA
≤ 100 µl SEC (centrifugation) EX03
≤ 150 µl SEC (fractionation by gravity flow) EX05
100 µl to 500 µl Precipitation + SEC (centrifugation) EX02
1 ml SEC (fractionation by gravity flow) EX04
PLASMA
≤ 150 µl SEC (fractionation by gravity flow) EX05
100 µl to 250 µl Precipitation + SEC (centrifugation) EX02
1 ml SEC (fractionation by gravity flow) EX04

* For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides.
† Highly concentrated exosome samples (e.g. 1x1012 particles/ml) other than blood can also be used.

EX02 Exo-spin™ kit comparative data

EX02 Exo-spin™ kit comparative data

EX02 Exo-spin™ kit comparative data

Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).

EX04 Exo-spin™ midi columns comparative data

EX04 Exo-spin™ midi columns comparative data

Comparison of total exosome recovery from 10 ml conditioned cell culture medium. The total particle yield obtained with Exo-spin™ buffer + Exo-spin™ midi columns is greater than the yield obtained using the membrane affinity method. Notably, Exo-spin™ buffer is much more efficient than generic PEG 6000 when used prior to Exo-spin™ midi column exosome isolation.

Downstream applications
• RNA studies
• Mass spectrometry
• Western blotting/ELISA
• Nano particle tracking and functional studies

Exo-spin™ citation list

Material available for download
Exo-spin™ User Guide
Application Note: Exosome isolation from CSF
Application Note: Comparison between Precipitation & Size Exclusion Chromatography (SEC) vs. Membrane Affinity for exosome isolation

Products

Note: availability depends on country - see product detail page.

Doaa Cell Guidance