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Routine cloning and library construction competent cells

The E. cloni® competent cells from Lucigen incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. Available in chemically and electrocompetent formats in a range of high transformation efficiencies, the E.cloni cells are ideal as direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10 and XL1-Blue).

Benefits of the E. cloni range
• High yield optimised
• Directly replace standard cloning strains
• phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable
• Range of high electro competent transformation efficiencies (5 x 109 to 4 x 1010 cfu/µg)
• Range of high chemically competent transformation efficiencies (1 x 106 to 1 x 109 cfu/µg)
 

Format Name Efficiency Applications
Electrocompetent E. cloni 10G SUPREME Electrocompetent Cells > 4 x 1010 cfu/µg pUC DNA SUPREME Cells are suitable for construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 x 1010 cfu/µg pUC DNA ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA.
E. cloni 10G CLASSIC Electrocompetent Cells > 5 x 109 cfu/µg pUC DNA These cells are the most economical choice for standard cloning and library construction.
Chemically competent E. cloni 10G Chemically Competent Cells > 1 x 109 cfu/µg pUC DNA Highly efficient competent cells for routine cloning applications. Available in 96-well well format for higher throughput.
E. cloni 10G Chemically Competent Cells, Subcloning Grade > 1 x 106 cfu/µg pUC DNA The best value for simple cloning and plasmid propagation.
E. cloni 5-alpha Chemically Competent Cells > 1 x 108 cfu/µg pUC DNA Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.


E. cloni citations

Material available for download
E. cloni competent cells summary
E. cloni 10G and 10GF´ electrocompetent cells manual
E. cloni 10G chemically competent cells manual
E. cloni 5-alpha chemically competent cells manual
E. cloni 96-well chemically competent cells manual

DH5α and DH10B are trademarks of Life Technologies Inc

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
E. cloni 10G Supreme Electrocompetent Cells 60081-1 · Lucigen
60081-1
Lucigen
12 reactions (solos) £481.00
12 reactions (solos)
view
E. cloni 10G Supreme Electrocompetent Cells 60080-1 · Lucigen
60080-1
Lucigen
12 reactions (duos) £423.00
12 reactions (duos)
view
E. cloni 10G Supreme Electrocompetent Cells 60080-2 · Lucigen
60080-2
Lucigen
24 reactions (duos) £776.00
24 reactions (duos)
view
E. cloni 10GF' Electrocompetent Cells 60061-1 · Lucigen
60061-1
Lucigen
12 reactions (duos) £277.00
12 reactions (duos)
view
E. cloni 10GF' Electrocompetent Cells 60061-2 · Lucigen
60061-2
Lucigen
24 reactions (duos) £474.00
24 reactions (duos)
view
E. cloni 10G Elite Electrocompetent Cells 60052-1 · Lucigen
60052-1
Lucigen
12 reactions (duos) £273.00
12 reactions (duos)
view
E. cloni 10G Elite Electrocompetent Cells 60052-2 · Lucigen
60052-2
Lucigen
24 reactions (duos) £467.00
24 reactions (duos)
view
E. cloni 10G Elite Electrocompetent Cells 60052-3 · Lucigen
60052-3
Lucigen
24 reactions (sixpacks) £361.00
24 reactions (sixpacks)
view
E. cloni 10G Elite Electrocompetent Cells 60052-4 · Lucigen
60052-4
Lucigen
48 reactions (sixpacks) £596.00
48 reactions (sixpacks)
view
E. cloni 10G Classic Electrocompetent Cells 60117-1 · Lucigen
60117-1
Lucigen
24 reactions (sixpacks) £321.00
24 reactions (sixpacks)
view
E. cloni 10G Classic Electrocompetent Cells 60117-2 · Lucigen
60117-2
Lucigen
48 reactions (sixpacks) £571.00
48 reactions (sixpacks)
view
E. cloni 10G Chemically Competent Cells 60107-1 · Lucigen
60107-1
Lucigen
12 reactions (duos) £201.00
12 reactions (duos)
view
E. cloni 10G Chemically Competent Cells 60107-2 · Lucigen
60107-2
Lucigen
24 reactions (duos) £353.00
24 reactions (duos)
view
E. cloni 10G Chemically Competent Cells, Subcloning Grade 60108-1 · Lucigen
60108-1
Lucigen
48 reactions £117.00
48 reactions
view
E. cloni 5-alpha Chemically Competent Cells 60602-1 · Lucigen
60602-1
Lucigen
12 reactions (duos) £182.00
12 reactions (duos)
view