NxGen Grade T7 RNA Polymerase

The NxGen® Grade T7 RNA polymerase from LGC Biosearch (Lucigen) is in a highly pure form (99% purity) specifically for next-generation applications. The T7 RNA polymerase catalyzes the 5′ to 3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.

Features of the NxGen T7 RNA Polymerase

Unit concentration	50,000 U/mL
Purity (SDS-PAGE)	>99%
SS exonuclease	500 U <0.1% released
DS exonuclease	500 U <0.1% released
Endonuclease	500 U <10% converted
RNAse contamination	500 U = none detected
E. coli 16S rDNA contamination	500 U <10 copies

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Material available for download
NxGen T7 RNA Polymerase datasheet

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
NxGen RNAse Inhibitor, 40 U/µL 30281-1 · Lucigen	30281-1 Lucigen	10000 units	£356.00 10000 units view
NxGen T7 RNA Polymerase, 50 U/µL 30223-1 · Lucigen	30223-1 Lucigen	25000 units	£237.00 25000 units view

In this section

NxGen T7 RNA Polymerase datasheet