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NxGen® Grade Phi29 DNA Polymerase

NxGen® Grade Phi29 DNA Polymerase

Cambridge Bioscience offers a highly processive DNA polymerase with powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. Capable of up to 70,000 insertions per binding event, the NxGen® phi29 DNA Polymerase is ideal for generating high levels of DNA whilst conserving sequence integrity.

Features of the NxGen® phi29 DNA polymerase
• Unit Concentration: 10,000 U/mL
• Purity (SDS-PAGE): >99%
• SS Exonuclease: Functional
• E. coli 16S rDNA Contamination: 100 U <10 copies
• Endonuclease: 100 U <10% converted
• Storage: -20°C
• Source: recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.

Material Available for Download
NxGen® phi29 DNA Polymerase Manual
NxGen® phi29 DNA Polymerase SDS

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