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NxGen® Grade Phi29 DNA Polymerases

NxGen® Grade Phi29 DNA Polymerases

Cambridge Bioscience offers the genomic-grade phi29 DNA polymerase in a highly pure form (99% purity) specifically for next-gen applications. phi29 DNA polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function, ensuring quality data and high reproducibility. The enzyme is capable of up to 70,000 insertions per binding event.

Features of the NxGen® phi29 DNA polymerase
• Unit Concentration: 10,000 U/mL
• Purity (SDS-PAGE): >99%
• SS Exonuclease: Functional
E. coli 16S rDNA Contamination: 100 U <10 copies
• Endonuclease: 100 U <10% converted
• Storage: -20°C
• Source: recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.

Applications
• Rolling circle amplification (RCA)
• Whole genome amplification (WGA)
• Multiple displacement amplification (MDA)
• Template preparation for SNP & STR genotyping
• Template preparation for NGS
• Single-cell DNA amplification (cell-free cloning)
• Signal amplification tool for detecting bacterial & viral DNA in clinical samples, proteins in solid-phase immunoassays, & biomarkers in tissue

Material Available for Download
NxGen® phi29 DNA Polymerase Manual
NxGen® phi29 DNA Polymerase SDS

Products

Note: availability depends on country - see product detail page.

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