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Rapid & Complete DNA Bisulphite Conversion Kits
High Quality DNA & RNA Purification From Agarose
Total RNA Purification From Acid-Guanidinium-Phenol Reagents

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

Modified Cytosine Optimised PCR Taq - QuestTaq™

Modified Cytosine Optimised PCR Taq - QuestTaq™
Cambridge Bioscience offers a Taq polymerase for the robust, non-biased amplification of DNA containing modified cytosine residues. Ideal for the amplification of 5-methylcytosine (5-mC), 5-hydroxymethylcytosine (5-hmC) and glucosyl-5-hydroxymethylcytosine (g5hmC) containing DNA, QuestTaq™ ensures high yield amplification across a wide range of templates.

Benefits of Using QuestTaq™
• One-tube PCR - Contains premixed reagents
• Robust amplificaion
• Non-biased amplification
• High yield amplification across a wide range of templates
• 5mC, 5hmC & g5hmC modified DNA amplified
• Ideal for real-time, quantitative & end-point analyses
• Compatible with a range of fluorescent dyes for use in real-time PCR

DNA Template Amplification Comparison Data
QuestTaq™ polymerase consistently yields robust amplicons from DNA templates having modified/unmodified cytosines. The level (intensity) of an ~900 bp product generated from DNA templates using QuestTaq™ PreMix or the polymerases from Suppliers X and Y. PCR products were separated in a 0.8% (w/v) agarose/TAE/EtBr gel. Lanes correspond to amplicons from template DNA containing: unmodified cytosine (c), 5-methylcytosine (m), 5-hydroxymethylcytosine (h), or glucosyl-5-hydroxymethylcytosine (g). (M) is a 1 kb DNA Marker (Cat. No. M5006-50).

Optimised Premixes
QuestTaq™ polymerase is supplied within two optimised premixes:

1. QuestTaq™ PreMix
QuestTaq™ PreMix is supplied as a convenient 2X concentrated “master mix” containing all the reagents (i.e., dNTPs, MgCl2, and enhancers) necessary for robust PCR with little or no by-product formation. QuestTaq™ PreMix excludes a DNA dye allowing it to be used with one of the researcher's own.

2. QuestTaq™ qPCR PreMix
QuestTaq™ qPCR PreMix also includes an intense dsDNA-specific fluorescent dye (SYTO® 9) for sensitive/precise DNA detection. QuestTaq™ qPCR PreMix is ideal for locus-specific 5-hmC detection when used in conjunction with the Quest 5-hmC Detection Kit™. To find out more, Click Here

Material Available for Download
QuestTaq™ PreMix Protocol
QuestTaq™ qPCR PreMix Protocol

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