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Mix-n-Stain Antibody Labelling Kits
mRNA Based Live Cell Staining Solution
Fixable & Non-Fixable Neuronal Terminal Dyes

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CF™594: The Brightest Deep Red Dye

CF™594: The Brightest Deep Red Dye
Cambridge Bioscience offers a deep red fluorescent dye which, when conjugated to proteins, is significantly brighter than Alexa Fluor® 594 due to the high quantum yield and water solubility. CF™594 has excellent photostability making it ideal for demanding applications like confocal microscopy and single molecule imaging as well as combining it with blue fluorescent CF™405 dyes, green fluorescent CF™488A and far red CF™640R for multi-colour imaging.

CF™594 Technical Details
• 593/614 nm Abs/Em Maxima
• 115,000 Extinction Coefficient
• ~730 Molecular Weight
• 532 nm, 568 nm or 594 nm Excitation Laser Line
• Direct Replacement For Alexa Fluor® 594, ATTO 594, DyLight™ 594 & Texas Red®

Benefits Of Using CF™594
• Brighter than Alexa Fluor® 594
• Excellent photostability

CF594 Relative fluorescence of CF™594 and Alexa Fluor® 594 (AF594) goat anti-mouse conjugates as a function of the number of dye molecules per protein (degree of labeling).

CF594 2Photostability of CF™594 and Alexa Fluor® 594 (AF594) goat anti-mouse conjugates. Intracellular staining of Jurkat cells was performed with mouse anti-CD3 followed by CF™594 or AF594 goat anti-mouse conjugates. Cells were continuously exposed to mercury arc lamp microscope excitation with a Texas Red® filter set. Images were captured every 15 seconds for 5 min and fluorescence intensity was normalized to time 0.

CF™ Dye Citation List

Alexa Fluor® and Texas Red® are registered trademark of Invitrogen
DyLight™ is a trademark of Thermo Fisher Scientific Inc.

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