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Mix-n-Stain Antibody Labelling Kits
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CF™488A: superior specific labelling

CF™488A: superior specific labelling

The CF™488A green fluorescent dye gives greater target specificity when bound to antibodies than those labelled with Cy™2 or Alexa Fluor® 488. CF™488A is less highly charged than Alexa Fluor® 488 therefore not distorting the protein isoelectric point and affinity, increasing the signal to noise ratio.CF™488A has similar brightness to Alexa Fluor® 488 whilst reducing spillover into the red channel.

CF™488A technical details
• 490/515 nm Abs/Em Maxima
• 70,000 extinction coefficient
• ~710 molecular weight
• 488 nm excitation laser line
• Direct replacement for Alexa Fluor® 488, DyLight™488, fluorescein (aka FITC, FAM) and Cy™2

Benefits of using CF™488A
• Antibody conjugates show increased sensitivity over competitors
• High photostability
• Water soluble and pH insensitive
• Reduced spillover into red channel compared to Alexa Fluor® 488

Staining Specificity ComparisonCF488A

Cryosections of human heart stained with rabbit anti-fibronectin followed by goat anti-rabbit IgG conjugated to Cy2, Alexa Fluor 488, and CF488A, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim, Hessen, Germany.

CF™488A formats available
• Labelled Secondary Antibodies - find out more.
• Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - find out more.

A small selection of the CF™488A products avilable are shown below. Alternatively, please use the website search to find the product of choice or contact our Biotium specialist for more information.

CF™ Dye citation list

Alexa Fluor® is a registered trademark of Invitrogen. 
DyLight™ is a trademark of Thermo Fisher Scientific Inc.
Cy™ is a trademark of GE Healthcare. 


Note: availability depends on country - see product detail page.

Speak To Our Biotium Specialist, Hiten