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Antibleaching Live Cell Visualisation Medium - DMEMgfp-2

Antibleaching Live Cell Visualisation Medium - DMEMgfp-2

Cambridge Bioscience offers an antibleaching live cell visualisation medium to vastly increase the stability of green fluorescent proteins (GFPs). DMEMgfp-2  excludes the components that can cause oxidative reddening of GFPs leading to up to a 9-fold increase of photostability of EGFP, a 3.3-fold increase of photostability of TagGFP2 and more than a 4-fold increase of photostability of activated forms of photoactivatable PA-GFP and PS-CFP2 (the effect varies for particular fusions and localisations). EGFP photostability can be further increased when rutin is added 30 minutes before imaging to DMEMgfp-2 results in further enhanced EGFP photostability (approximately 1.5-fold).

Workflow
Simply replace the standard cell culture medium with DMEMgfp-2 20-30 minutes before starting the imaging experiment under sterile conditions (or supplemented with rutin at a final concentration of 20 mg/l). This medium can be supplemented with L-glutamine, penicillin, streptomycin and fetal bovine serum. This medium is intended for imaging use and not for long term cell culture.

Benefits Of Using This DMEMgfp-2
• Up to 9-fold increase of photostability of EGFP
• Up to 3.3-fold increase of photostability of TagGFP2
• More than 4-fold increase of photostability of activated forms of photoactivatable PA-GFP and PS-CFP2

               
HeLa cells transfected with fluorescent protein-tagged α-tubulin or β-actin had a normal cytoskeleton after 5-day culture in DMEMgfp. Fluorescence microscopy of TagGFP2-α-tubulin and mKate2-β-actin of live HeLa cells after 5 day culturing in DMEMgfp.

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