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High Quality DNA & RNA Purification From Agarose
Quick, High Quality Total DNA Purification Kits
Request a Free Direct-zol RNA Miniprep Kit Sample

Transfection Ready Plasmid DNA In 20 Minutes With ZymoPURE II

Direct RNA Purification From Samples in TRI-Reagent® - Direct-zol™

Direct RNA Purification From Samples in TRI-Reagent® - Direct-zol™

Cambridge Bioscience offers the Direct-zol™ RNA kits for the quick and simple, chloroform and phenol free, spin column purification of high-quality total RNA directly from TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, RNA-Bee™ and similar acid-guanidinium-phenol based reagents. These kits enable the effective purification of up to 100 µg of high-quality total RNA with just 7 minutes of preparation time and without phase separation or precipitation.

Benefits of Using Direct-zol™ RNA MiniPrep Kits
• Consistent & efficient purification of small & large RNAs
• DNA-free RNA produced
• 7 minute preparation time
• No phenol or chloroform use
• Phenol carryover eliminated
• No phase separation, precipitation or post purification
• Wide sample compatibility
• Compatible with most RNA isolation reagents

Testimonials
"Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. I tried the Zymo kit and I was definitely hesitant and did not have high hopes for it. I was wrong.....the Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench. Thank you Zymo!"
A. Newhart (The Wistar Institute)

About Direct-zol™ RNA Kits

Product Binding capacity Format Elution volume Cells Tissue Protocol SDS
Direct-zol™ RNA Microprep 10 µg Spin-Column 6 µl ≤1x10 6 ≤5 mg Protocol SDS
Direct-zol™ RNA Miniprep 50 µg Spin-Column 25 µl ≤5x10 6 ≤25 mg Protocol SDS
Direct-zol™ RNA Miniprep Plus 100 µg Spin-Column 50 µl ≤1x10 7 ≤50 mg Protocol SDS
Direct-zol™-96 10 µg 96-Well Plate 10 µl ≤1x10 6 ≤5 mg Protocol SDS
Direct-zol™-96 MagBead 10 µg Magnetic Beads 50 µl ≤1x10 6 ≤5 mg Protocol SDS

 

  Direct-zol™ Trizol method Supplier A miRNA kit Supplier Q phenol kit
Time per prep 7 min 90+ min 30 min 30 min
No phase separation x x x
NGS-grade x x x
Small RNA recovery x x
DNase I included x x x

 

Obtain NGS-Ready RNA With Direct-zol™

NGS-Ready DNA With Direct-zol™


High Quality Broad Range RNA Purified With Direct-zol™

DNA-free RNA purified from human epithelial cells using Direct-zol™ compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). Small RNAs effectively recovered with Direct-zol™ while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown) (green).

High Sensitivity Viral RNA Detection Following Direct-zol™ Isolation
Direct-zol™ method significantly improves the detection of West Nile virus when compared to the conventional phase separation method. The RT-qPCR data show ?Ct = 5 (average of two independent experiments). RNA was isolated from cell-free samples inactivated using

Material Available To Download
Direct-zol™ MiniPrep Plus User Manual
Direct-zol™ MicroPrep User Manual
Direct-zol™ MiniPrep User Manual

Free Samples
Request a free Direct-zol™ RNA MiniPrep Plus kit sample Here

Direct-zol™ Citation List

TRI Reagent®, TRIzol®, RNAzol® are registered trademarks of Molecular Research Center, Inc.
QIAzol® is a registered trademark of QIAGEN GmbH
TriPure™ is a trademark of Roche Diagnostics Operations, Inc.
RNA-Bee™ is a trademark of Tel-Test, Inc.

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