- In this section:
- EvaGreen manual
- EvaGreen citations
EvaGreen® from Biotium is a high performance, stable, non-mutagenic, non-cytotoxic DNA binding dye, ideal for use in in quantitative real-time PCR (qPCR) and a range of other applications. This highly sensitive dye is superior to many other commercially available PCR and HRM dyes.
Benefits of using EvaGreen
• Highly sensitive
• Environmentally safe, nonmutagenic and noncytotoxic
• Superior for qPCR and isothermal amplification
• Thermostable
• Hydrolytically stable
• Photostable
• Low PCR inhibition
• Compatible with fast PCR Protocols
• Compatible with multiplex PCR
• Compatible with various digital PCR platforms
EvaGreen dye is less inhibitory than SYBR® green, allowing for a much brighter signal
A comparison of the raw fluorescence signal from qPCR reactions performed with two EvaGreen master mixes (Forget-Me-Not™ EvaGreen and Fast EvaGreen) and QuantiNova® SYBR® Green.
EvaGreen Dye is less inhibitory to PCR than SYBR® Green, allowing you to use more dye, and thus achieve a stronger melt curve signal
Melt curve analysis of a GAPDH qPCR product using either an EvaGreen master mix (Forget-Me-Not EvaGreen) or a SYBR® Green master mix (QuantiNova® SYBR® Green)
EvaGreen Portfolio
1. Fast EvaGreen qPCR Master Mix
Fast Evagreen qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. Combining the EvaGreen qPCR dye and the Cheetah™ Taq hotstart DNA polymerase, superior results are deivered when compared to other commercial master mixes.
2. Fast-Plus EvaGreen qPCR Master Mix
The Fast-Plus EvaGreen qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Available in three formats containing different amounts of ROX reference dye for use in different PCR instruments.
3. Forget-Me-Not EvaGreen qPCR Master Mix
Forget-Me-Not EvaGreen qPCR Master Mixes are hot-start EvaGreen dye-based master mixes for use in real-time PCR applications and DNA melt curve analysis. Containing a low concentration of inert blue dye allowing you to easily see which wells contain the master mix, the Forget-Me-Not EvaGreen qPCR Master Mixes can be used in place of any SYBR® Green dye-based master mixes.
Applications
• qPCR
• DNA melt curve analysis
• Isothermal amplification
• Capillary gel electrophoresis
• DNA quantification in solution
• Selective dead cell detection in cell viability tests
Material available for download
EvaGreen manual
Fast EvaGreen qPCR master mix manual
Fast plus EvaGreen qPCR master mix manual
EvaGreen citations
SYBR is a registered trademark of Thermo Fisher Scientific Inc.
QuantiNova is a registered trademark of Qiagen GmbH
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| EvaGreen® 20X in Water, 1 mL BT31000-T · Biotium | BT31000-T Biotium |
1 ml |
£67.00
1 ml
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| EvaGreen®, 20X in Water, 5x1 mL BT31000 · Biotium | BT31000 Biotium |
5 x 1 ml |
£293.00
5 x 1 ml
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| Fast EvaGreen® Master Mix for qPCR, trial size (100 rxn) BT31003-T · Biotium | BT31003-T Biotium |
100 reactions |
£102.00
100 reactions
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| Fast EvaGreen® Master Mix for qPCR(200 rxn) BT31003 · Biotium | BT31003 Biotium |
2 x 1 ml |
£213.00
2 x 1 ml
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| Fast EvaGreen® Master Mix for qPCR (500 rxn) BT31003-1 · Biotium | BT31003-1 Biotium |
5 x 1 ml |
£468.00
5 x 1 ml
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| Fast EvaGreen® Master Mix for qPCR (5000 rxn) BT31003-2 · Biotium | BT31003-2 Biotium |
50 x 1 ml |
£4262.00
50 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with low Rox (trial size, 100 rxn) BT31014-T · Biotium | BT31014-T Biotium |
1 ml |
£117.00
1 ml
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| Fast Plus EvaGreen® Master Mix for qPCR with low Rox (200 rxn) BT31014 · Biotium | BT31014 Biotium |
2 x 1 ml |
£249.00
2 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with low Rox (500 rxn) BT31014-1 · Biotium | BT31014-1 Biotium |
5 x 1 ml |
£540.00
5 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with low Rox (5000 rxn) BT31014-2 · Biotium | BT31014-2 Biotium |
50 x 1 ml |
£4902.00
50 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with High Rox (trial size, 100 rxn) BT31015-T · Biotium | BT31015-T Biotium |
1 ml |
£117.00
1 ml
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| Fast Plus EvaGreen® Master Mix for qPCR with high Rox (200 rxn) BT31015 · Biotium | BT31015 Biotium |
2 x 1 ml |
£249.00
2 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with high Rox (500 rxn) BT31015-1 · Biotium | BT31015-1 Biotium |
5 x 1 ml |
£540.00
5 x 1 ml
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| Fast Plus EvaGreen® qPCR Master Mix with high Rox (5000 rxn) BT31015-2 · Biotium | BT31015-2 Biotium |
50 x 1 ml |
£4902.00
50 x 1 ml
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| Forget-Me-Not™ EvaGreen® qPCR Master Mix (100 reactions) BT31041-T · Biotium | BT31041-T Biotium |
1 ml |
£91.00
1 ml
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