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Active Endosome Escape Based Transfection Reagent
Breast Cancer Cell Optimisesd Transfection Reagent

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E. cloni® 5-α Chemically Competent Cells

E. cloni® 5-α Chemically Competent Cells

The E. cloni® 5-α Chemically Competent Cells are versatile chemically competent cells ideal for routine cloning, subcloning, and plasmid isolation with or without blue/white screening. Containing the most useful genetic elements of standard cloning strains, the E. cloni® 5-α Chemically Competent Cells provide high yields and high quality plasmid DNA.

Benefits of using the E. cloni® 5-α Chemically Competent Cells
• High yield optimised
• Wide range of applications
• Direct replacements for standard cloning strains

E. cloni® 5-α Chemically Competent Cells
• Transformation efficiency: over 1×108 cfu/µg DNA (cfu/µg pUC DNA)
• Cloning methylated DNA: no
• BAC, cosmid cloning: no
• Blue/white screening: yes, no IPTG induction required

Genotype:
fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1hsdR17

Custom competent cells
We offer access to a custom competent cell service for general cloning, large or difficult fragment cloning, library construction, protein expression and phage display library (ER2738, SS320, TG1 strains available) applications. Find out more here.

Material available for download
E. cloni® 5-alpha Chemically Competent Cells manual

Products

Note: availability depends on country - see product detail page.

Contact Our Lucigen Specialist, Doaa