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Product types:
Competent Cells Eliminating Endotoxin At Source
Breast Cancer Cell Optimisesd Transfection Reagent
Electrocompetent Cells With The Highest Transformation Efficiency

Take The DeNovix Challenge

E. cloni® 10G Chemically Competent Cells

E. cloni® 10G Chemically Competent Cells

These high efficiency chemically competent cells offer excellent performance. The E. cloni® Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109 and TOP10 and are able to directly replace them in cloning protocols.

Benefits of using the E. cloni® 10G Chemically Competent Cells
• High yield optimised
• Directly replace standard cloning strains
• Range of high transformation efficiencies available (1 × 106 to 1 x 109cfu/µg)

 E. cloni® 10G competent cell applications
• Routine cloning, subcloning and plasmid isolation with or without blue/white screening.

Choice of efficiency:
E. cloni® 10G Chemically Competent Cells
(More than 1 × 109 cfu/µg pUC DNA; (more than 1 × 108 in 96-well plates)
Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates for higher throughput.
E. cloni® 10G Chemically Competent Cells
Subcloning Grade (> 1 × 106 cfu/µg pUC DNA)
The best value available anywhere for simple cloning and plasmid propagation.

E. cloni 10G genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA

E. cloni® Electrocompetent Cells
Find out about electrocompetent versions of 10G here

Custom Competent Cells
We offer access to a custom competent cell service for general cloning, large or difficult fragment cloning, library construction, protein expression and phage display library (ER2738, SS320, TG1 strains available) applications. Find out more here

Free samples
Request a free sample of E. cloni® 10G chemically competent cells here

Material available for download
E. cloni® 10G and 10GF’ Chemically Competent Cells manual
E. cloni® 96-well Chemically Competent Cells manual
E. cloni® Competent Cells flyer

DH5α™ and DH10B™ are trademarks of Invitrogen Corp.


Note: availability depends on country - see product detail page.

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