COX-1 & COX-2 activity assays

These assays from Cayman Chemical are highly sensitive colourimetric and fluorometric COX activity assays. Both assays are suitable for examining COX activity in crude cell lysates, tissue homogenates and purified enzyme preparations and are supplied with COX-1 and COX-2 specific inhibitors to enable the specific measurement of either isoform.

Benefits of using COX activity assays
• Highly sensitive and precise
• Enables assaying of specific COX-1 or COX-2 activity
• Compatible with crude cell lysates, tissue homogenates and purified enzyme preparations
• Tested negatively for interference with a wide range of common substances

Colourimetric COX activity assay kit specifications (CAY760151-96 wells)
• 13-63 nmol/min/ml sensitivity
• Precision - intra-assay: 2.6% and inter-assay: 5.4 %
• Peroxidase activity assayed colorimetrically by monitoring appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm
• Assay includes COX-1 and COX-2 specific inhibitors to distinguish between the two isozymes

Fluorescent COX activity assay kit specifications (CAY700200-2 x 96 wells)
• 0.023-2 nmol/min/ml sensitivity
• Precision - intra-assay: 2.2% and inter-assay: 2.3 %
• Plate-based, fluorometric measurement (ex 530-540 nm, em 585-595 nm)
• Utilises reaction between PGG2 and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) which produces highly fluorescent compound resorufin
• Kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity




Material available for download
COX Fluorescent Activity Assay Kit Protocol
COX Activity Assay Kit Protocol