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Supplier information: Biotium

Biotium offers a variety of fluorescent reagents and related biochemicals that find applications in genomics, proteomics, molecular biology, cell biology, neuroscience and drug screening.

We are delighted to offer the revolutionary new Mix-n-Stain™ CF™ Dye Antibody Labelling Kit from our partner Biotium.

With 16 dye options spanning absorbances of 350nm to 770nm and less than 30 seconds hands on time, this kit will dramatically simplify the process of preparing fluorescently labelled antibodies producing a conjugate ready for microscopy, immunofluorescence, flow cytometry or Western blotting.

Tto find out more, please Click Here

Fast EvaGreen® qPCR Master Mix is a PCR reagent suitable for both qPCR using a fast cycling protocol and high resolution melt curve (HRM) analysis. The reagent system uses two breakthrough PCR technologies from Biotium, EvaGreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

• Superior sensitivity and wide dynamic range
• Suitable for both qPCR and HRM
• Compatible with both fast and regular cycling protocols
• Novel chemically-modified hotstart Taq requiring only 2 minutes to activate

For further information please click here

Modified using a novel modifying reagent, Cheetah™ Taq has been improved upon AmpliTaq Gold and other similar hot-start enzymes by having a faster activation time and better shelf-life. Under standard hot-start conditions (i.e., 94 °C in pH 8-9 Tris), Cheetah™ Taq regains both the 5'-3' polymerase and 5'-exonuclease activities within two minutes. Cheetah™ Taq is also superior to antibody-based hot-start DNA polymerases due to its complete supression of enzyme activity at room temperature and nearly full recovery of enzyme activity following activation. In addition, Cheetah™ Taq is also significantly more economical to manufacture than antibody-based hot-start enzymes.

Cheetah™ Taq is a patent-pending invention completely independent of AmpliTaq Gold and related technologies.  
For the protocol please click here

Cheetah Taq price
If you are looking for a safe alternative to Ethidium Bromide, without compromising on performance, then you should try GelRed™ or GelGreen™ nucleic acid stains. These gels stains are widely recognised as the best alternatives to Ethidium Bromide for the following reasons:
  • Unrivalled sensitivity
  • Cell membrane impermeability for true safety. Impenetrable to latex gloves
  • Compatible with all downstream manipulations
  • Extremely stable to both heat and light
  • Compatible with existing instruments
The products have recently been updated to a new convenient and efficient Prestaining Kit format. More information can be found here.

AccuBlue kits (formally MagicBlue) are superior DNA quantitation kits offering tremendous convenience, sensitivity, and wide dynamic range. Key features of AccuBlue kits include:

 BT31006 AccuBlue High  sensitivity dsDNA Quantitation Kit BT31007  AccuBlue Broad Range dsDNA Quantitation Kit
Sensitivity  

detect as little as 200 pg dsDNA per well

 

detect as little as 2 ng per well

Wide linear detection range Quantify dsDNA from 0.2 to 100 ng   Quantify dsDNA from 2 to 2000 ng
Compatibility compatible with microplate readers or the Qubit™ fluorometer from Invitrogen   compatible with microplate readers
Excitation / Emission 485nm/530nm   350nm/460nm 

Benefits

  • Safety: kit is cell membrane impermeable to eliminate or reduce any potential   genotoxicity
  • Excellent selectivity: minimally affected by RNA or ssDNA and many other common contaminants
  • Superior precision: less than 5% CVs
  • Unmatched stability and convenience: contain a stable 1X aqueous solution, 100X enhancer and dsDNA standards for use at anytime


We also offer trial sizes of the AccuBlue dye solution, cat BT31008-T (for high sensitivity solution) and  BT31009-T (for broad range solution), enough solution to do two 96-well plates. 

Protocol for BT31006
Protocol for BT31007

AccuBlue Kits and Solutions
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