your link to a world of discovery

Supplier information: Lucigen

Lucigen focus on developing new, much more effective products and technologies for gene cloning and genomics. Lucigen's patented CloneSmart® technology dramatically improved DNA cloning reliability and efficiency, allowing the successful cloning of genes that had been impossible to clone with standard methods. Since CloneSmart, Lucigen have developed and introduced many other cloning breakthroughs including ClonePlex®-AK dual insert cloning kits, CopyRight® large insert cloning kits, UltraClone™ DNA ligation & cloning kits, and BigEasy® linear cloning kits.

The Fastest and Easiest PCR-based Cloning and Expression System

The Expresso™ T7 Cloning and Expression System from Lucigen improves upon the most powerful technique for recombinant protein expression. Expresso™ cuts the traditional 10 hour multistep pET T7 process down to just 3 hours and 3 easy steps; PCR amplification, mixing and transformation.

Benefits include:

Enzyme-free cloning in seconds
Ideal for expressing toxic proteins
Easy Purification: Choice of N or C-terminal 6xHis tagged proteins
Cloning-ready vector- no vector preparation needed

Two sets of optimised competent cells are included in these kits for Cloning and Expression.

For further details click here

Expresso™ T7 Cloning and Expression Products

Clone the Unclonable

Lucigen's pSMART vectors are a new generation of transcription and translation-free cloning vectors designed to eliminate cloning gaps commonly found with many plasmids.

Gap-free cloning
<1% background
No post-ligation cleanup prior to transformation
Small vector size (1.7-2.0kb)
Ampicillin or kanamycin selection
Pre-processed vectors eliminate vectro digestion, gel purification and dephosphorylation
High efficiency cloning with either electroporation or chemically competent cells

click here for more information

Big Easy Long PCR Cloning Kit

The BigEasy Long PCR Cloning Kits contain everything needed to efficiently clone PCR products up to 30 kb into an unbiased, high-fidelity cloning vector. There are two different versions of the kit that are compatible with either proofreading or non-proofreading PCR polymerases. They can also be used to clone any blunt or G-tailed DNA up to 30 kb.