7-AAD (7-amino-actinomycin D) is used as a viability probe for methods of nonviable cell exclusion. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter). Cambridge Biosciences offers a number of 7-AAD products.
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