Triglyceride Colorimetric Assay Kit

Cayman’s Triglyceride Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying mono-, di-, and triglycerides in plasma, serum, cell culture extracts, and tissue homogenates. The Triglyceride Colorimetric Assay Kit uses the enzymatic hydrolysis of triglycerides by lipase to produce glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system. The glycerol formed in reaction 1 is phosphorylated to glycerol-3-phosphate in a reaction catalyzed by glycerol kinase. The glycerol-3-phosphate is oxidized by glycerol phosphate oxidase producing dihydroxyacetone phosphate and hydrogen peroxide (H2O2). Peroxidase catalyzes the redox-coupled reaction of H2O2 with 4-aminoantipyrine (4-AAP) and N-ethyl-N-(3-sulfopropyl)-m-anisidine (ESPA), producing a brilliant purple color. The absorbance is measured at 540 nm.