DiSC2(5) (3,3'-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization.
- &lamba;Abs (pH 7.5 buffer) = 646 nm (free dye); ~586 nm (dsDNA); ~531 nm (PNA-DNA)
- Dark solid soluble in DMSO or MeOH
- Store at 4°C
- C23H23IN2S2
- MW: 518.48
- Data sheet: View or download
Storage Temperature:
R