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DAPI (4',6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate, a more water soluble form of the dye.
- Classic blue nuclear counterstain for fixed cells
- Can be used at higher concentrations to stain live cells
- &lamba;Ex/&lamba;Em = 358/461 nm (with DNA)
- Off-white solid soluble in H2O
- Store at 4°C and protect from light, especially in solution
- C16H17Cl2N5
- MW = 350.25
- [28718-90-3]
- Data sheet: View or download
Storage Temperature:
R