DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate)

DAPI is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. DAPI dilactate is essentially the same as DAPI dihydrochloride, but with a different counterion. The dilactate salt form of the dye is more water soluble than the dihydrochloride salt, and therefore DAPI dilactate is a better choice for preparing stock solutions in water.   

 
• Classic blue nuclear counterstain for fixed cells
 
• Can be used at higher concentrations to stain live cells
 
• &lamba;_Ex/&lamba;_Em(DNA) = 358/461 nm
 
• Yellow solid soluble in water
 
• Store at 4°C and protect from light, especially in solution
 
• C₂₂H₂₇N₅O₆
 
• MW: 457.49
 

• Data sheet: View or download