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CF®405M Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

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500 µl
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This product is prepared by labeling highly cross-adsorbed goat anti-mouse IgG (H+L) with CF®405M dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405M dye has excitation/emission at 408/452 nm. Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. To minimize crossreactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep serum.

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image 1
MCF-7 cells stained with mouse anti-pPol
MCF-7 cells stained with mouse anti-pPol-II and CF405S goat anti-mouse (nuclei, blue) and rabbit anti-COXIV and CF640R goat anti-rabbit (mitochondria, red).
MCF-7 cells stained with mouse anti-tubu
MCF-7 cells stained with mouse anti-tubulin and CF405M goat anti-mouse (microtubules, cyan). Nuclei are stained with RedDot2 (red).