Quest 5-hmC Detection Kit w/ Zymo-Spin™ I Columns (Uncapped)
The Quest 5-hmC Detection Kit™ from Zymo Research allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient reaction setup. Utilizing a robust and highly specific 5-hmC Glucosyltransferase enzyme, 5-hydroxymethylcytosine in DNA is specifically tagged with a glucose moiety yielding glucosyl-5-hydroxymethylcytosine. Following glucosylation of 5-hydroxymethylcytosine, digestion of DNA with “glucosyl-5-hydroxymethylcytosine sensitive” restriction endonucleases (GSRE) allows for effective differentiation of 5-methylcytosine from 5-hydroxymethylcytosine. Identification of 5-hydroxymethylcytosine in a sequence specific context can then be deduced from the restriction endonuclease recognition sequence. The Quest 5-hmC Detection Kit™ contains the GSRE Glal. Glal can digest DNA only when 5-methylcytosine or 5-hydroxymethylcytosine lies within its recognition sequence. When 5-hydroxymethylcytosine is glucosylated (glucosyl-5-hydroxymethylcytosine), GlaI is no longer able to digest.
-70C / RT