Cultured rat retinal ganglion cells were stained with anti-Thy-1 and anti-neurofilament-L followed by secondary antibodies and DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Pang I, Zeng H, Fleenor DL, Clark AF. Pigment epithelium-derived factor protects retinal ganglion cells. BMC Neurosci. 2007;8:11. Figure 1(d)
Human retinal pigmented epithelial cells were stained with 546 labeled phalloidin and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
Frozen I4895T mouse soleus muscle section was stained with PROTEOSTAT® aggresome detection reagent and Hoechst 33342 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Lee CS, Hanna AD, Wang H, Dagnino-Acosta A, Joshi AD, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659. Figure 6(a)
PROTEOSTAT® is a registered trademark of Enzo Life Sciences, Inc.
Human retinal pigmented epithelial cells were stained with anti-β-tubulin followed by an AF488 secondary antibody and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Hua K, Ferland RJ. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5. Figure 1
MDA-MB-231 cells were stained with conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from McConnell BV, Koto K, Gutierrez-Hartmann A. Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression. Mol Cancer. 2011;10:75. Figure 5
Frozen mouse circumvallate papillae section was stained with anti-IP3R3 followed by a Rhodamine Red™-X secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Clapp TR, Stone LM, Margolskee RF, Kinnamon SC. Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction. BMC Neurosci. 2001;2:6. Figure 4(b)
Rhodamine Red™ is a trademark of Thermo Fisher Scientific, Inc.
Frozen Dcc+/+ mouse lumbar dorsal root ganglion section was stained with anti-CGRP, anti-isolectin B4, and anti-Substance P followed by AF488, AF555, and AF647 conjugated secondary reagents and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Liang D, Zheng M, Sun Y, Sahbaie P, Low SA, Peltz G, et al. The Netrin-1 receptor DCC is a regulator of maladaptive responses to chronic morphine administration. BMC Genomics. 2014;15:345. Figure 5(a)
GFP-prestin/Xpress-tagged Fabp3 co-transfected opossum kidney cells were stained with anti-Xpress followed by an AF546 conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Zheng J, Anderson CT, Miller KK, Cheatham M, Dallos P. Identifying components of the hair-cell interactome involved in cochlear amplification. BMC Genomics. 2009;10:127. Figure 5(c)
Whole mount Drosophila larva testis was stained with anti-DSXM, anti-TJ, and anti-EYA followed by AF488 and AF647 conjugated secondary antibodies and tyramide signal amplification CY3 system and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Hempel LU, Oliver B. Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells. BMC Dev Biol. 2007;7:113. Figure 7(d)
Whole S. littoralis ovariole was stained with anti-YP2 followed by an AF594 conjugated secondary antibody, Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Bebas P, Kotwica J, Joachimiak E, Giebultowicz JM. Yolk protein is expressed in the insect testis and interacts with sperm. BMC Dev Biol. 2008;8:64. Figure 4(b)
Apchu745 mutant zebrafish embryo brain section was stained with anti-BrdU followed by an AF647 conjugated secondary antibody, TO-PRO-3, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Lin J, Wang X, Dorsky RI. Progenitor expansion in apc mutants is mediated by Jak/Stat signaling. BMC Dev Biol. 2011;11:73. Figure 2(c)
Frozen mouse embryo jugular lymph sac section was stained with anti-Prox1 and anti-LA102 followed by AF488 and AF594 conjuagted secondary antibodies and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Buttler K, Ezaki T, Wilting J. Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43. Figure 1(d)
Paraffin embedded magnetically aggregated hepatocyte spheroid section was stained with anti-MRP2 followed by an AF488 conjugated secondary antibody, DAPI, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Desai PK, Tseng H, Souza GR. Assembly of hepatocyte spheroids using magnetic 3D cell culture for CYP450 inhibition/induction. Int J Mol Sci. 2017;18:1085. Figure 2(b)
Apoptosis in frozen mouse retinal sections post injection of sham, BENAQ or after DNAse I treatment was detected with terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Tochitsky I, Trautman J, Gallerani N, Malis JG, Kramer RH. Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Sci Rep. 2017;7:45487. Figure 4(a)
Frozen bitransgenic MTB/TRAS mouse mammary tissue section was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 then mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
Frozen bitransgenic MTB/TRAS mouse mammary tissue section 96 hr post administration of doxycycline was stained with anti-cytokeratin 8 and anti-Smad4 followed by AF488 and AF567 conjugated secondary antibodies and Hoechst 33258 and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Liu Z, Wang M, Alvarez JV, Bonney ME, Chen C, D'Cruz C, et al. Singular value decomposition-based regression identifies activation of endogenous signaling pathways in vivo. Genome Biol. 2008;9:R180. Figure 5(a)
Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 2(b)
Whole mount Symsagittifera roscoffensis embryo was stained with AF488 conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool. 2008;5:14. Figure 3(a)
Whole mount Wirenia argentea larva was stained with anti-acetylated-α-tubulin followed a FITC conjugated secondary antibody and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Todt C, Wanninger A. Of tests, trochs, shells, and spicules: Development of the basal mollusk Wirenia argentea (Solenogastres) and its bearing on the evolution of trochozoan larval key features. Front Zool. 2010;7:6. Figure 5(a)
Whole mount Hispolia malayensis zooid was stained with an AF488 conjugated phalloidin followed by DAPI and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Image from Schwaha T, Wood TS, Wanninger A. Myoanatomy and serotonergic nervous system of the ctenostome Hislopia malayensis: evolutionary trends in bodyplan patterning of ectoprocta. Front Zool. 2011;8:11. Figure 3(b)
