Transiently Transfected Cell Line Development
The construction and validation of stable cell lines is very labour-intensive, time-intensive, and cost-intensive. For ion channel cell lines, many functional channels are heteromers of multiple pore-forming and modulatory subunits. Coexpression necessitates the use of multiple antibiotic selection markers often resulting in unhealthy, slow-growing cells. Expression can be lost over time so the critical decision to make an inducible or constitutive expressing-cell line is dependent upon ion channel type. Transiently transfected cells can be a viable alternative to stable cell line production, but conventional transfection methods are not easily scalable for high-throughput screening applications.
Cambridge Bioscience offers access to a transiently transfected cell line development service from ChanTest.
ChanTest utilises a scalable electroporation system to efficiently deliver cDNA into cells eliminating many of the complications associated with viral vectors or membrane-modifying reagents. With this method, cDNAs encoding principal and auxiliary ion channel or GPCR subunits can be cotransfected to recapitulate functional characteristics.
Benefits & Features of Transiently Transfected Cell Line Development
• Cost savings - Compared to the development of stable cell lines
• Time savings - Compared to the development of stable cell lines
• Multiple subunit expression
• Scalable - Readily scalable for HTS
• High transfection efficiency
• Cryopreservation of assay-ready cells
• Single, quality-controlled batches provided for day-to-day assay consistency
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To discuss a transiently transfected cell line development project, please contact our experienced team by Clicking Here