Total RNA qPCR QuantificationTraditionally protein assembly has been considered the primary biological purpose of RNA. Ribosomal RNA and transfer RNA were two exceptions to this paradigm, which did not code for proteins. However today we now know there are numerous non-coding RNA types such as miRNA, snRNA, snoRNA, lncRNA and rRNA.
Most cDNA synthesis kits allow quantification of only one or two classes of RNA. However Cambridge Bioscience offers RNA Quant™, a cDNA synthesis and real-time qPCR kit that enables researchers to accurately measure several classes following a single synthesis reaction.