Single Base Resolution DNA Methylation Analysis
Cambridge Bioscience offers access to the single base resolution DNA methylation analysis service, EpiQuest™. EpiQuest™ is a genomic, single base resolution DNA methylation analysis service from Zymo Research. This services combines next-gen sequencing and innovative bioinformatics with well-established bisulfite technologies providing the most comprehensive DNA methylation analysis service available. This service is available in both a genome wide and whole genome format.
EpiQuest™ Genome Wide Service
The EpiQuest™ genome wide service uses a developed version of the conventional reduced-representation bisulfite sequencing (RRBS) method to increase sequence coverage. Genomic DNA is digested by restriction endonuclease enzymes to produce fragments rich in CpG sites. These fragments are recovered, subject to bisulfite conversion, and sequenced to give single nucleotide resolution sequencing data. This provides a statistically significant representation of CpG dinucleotides throughout the genome. Through the utilization of a multiple enzyme digestion and an optimized workflow, coverage has been expanded to > 75% of gene promoters and > 80% of CpG islands (for human samples).
EpiQuest™ Whole Genome Service
The EpiQuest™ whole genome Service utilizes a streamlined library preparation and sophisticated alignment algorithms to deliver whole genome bisulfite-sequencing that covers > 80% of all the bases in the human genome at single-base resolution.
The EpiQuest™ genomic services include:
• Genomic DNA Sample Validation
• Sample Processing & Library Preparation
• Next-Gen Sequencing
• Sequencing Validation & Bioinformatic Analysis
• Data Output & Delivery
There are three service options available
1. Full Service
The full service package removes undertakes the whole process of methylome sequencing. Customers simply submit DNA samples and receive high-quality data after comprehensive bioinformatic analysis. This package is ideal for customers without an extensive bioinformatics background
The following data is provided as part of the full service:
• Genome Browser Tracks
• Sequence Coverage Information
• Hierarchical Clustering
• Methylation Frequency
• Methylation Index
• Differentially Methylated Regions
• Correlation Data
2. Basic Service
The basic service package provides Next-Gen sequencing data aligned to the reference genome using Zymo Research's proprietary pipeline and three UCSC genome browser tracks. This option is ideal for users with extensive bioinformatic experience.
3. Customizable Service
The EpiQuest™ service can be completely customizable and adapted to fit the needs of the researcher. There are many options available for difficult samples including extra bioinformatic analysis, locus-specific validations, different organisms, and more.
Benefits of using the EpiQuest™ Service
• Streamlined workflows with comprehensive bioinformatic analysis
• Provides high quality data
• Low DNA input - < 500ng genomic DNA
• Applicable to a broad range of sample sources (human, mouse, rat, plant)
• Customizable service
• Rapid turnaround time
• Affordable cost
Request A No Obligation Quote
For further information or for a full quote, please contact us our experienced team by email or by phone (01223 316 855)