Random Shear BAC Library Construction
The constructon of BAC (Bacterial Artificial Chromosome) libraries is far more challenging than making typical, small-insert plasmid libraries due to the very large insert sizes. Even if successful, conventional BAC libraries are inherently biased and incomplete, due to non-random methods of DNA preparation and problems with conventional cloning vectors.
Cambridge Bioscience offers access to a random shear custom BAC library construction service from Lucigen. Utilising both the random shear technology and CloneSmart® transcription-free vectors, this service produces unbiased BAC libraries with exceptionally large inserts.
Benefits & Features of the Random Shear Technology & CloneSmart® Transcription-Free Vectors
There are numerous benefits provided by the combination of the exclusive random shear technology and the CloneSmart® transcription-free vectors offered by Lucigen.
• Unbiased BAC Libraries
This combination of technology eliminates the bias in DNA preparation and cloning. Clone gaps and sequencing gaps are practically eliminated and the potential problem of generating chimeric clones is eliminated.
• Large BAC Inserts Available
Conventional BAC libraries have a limited average insert size. The average insert size provided with this service is up to 100 kb or larger
• Exceptional Yield & Small DNA Input Required
Conventional BAC cloning requires high concentration and microgram quantities of purified large-insert DNA for ligation and packaging. High efficiency CopyRight v2.0 FOS vectors and the 10G BAC-Optimized Replicator™ v2.0 Competent Cells substantially increase recombinant yields produced by this service.
• Simple & Fast
This service enables the simple and quick production of BAC libraries
Request A No Obligation Quote
To discuss a random shear custom BAC library construction project, please contact our experienced team by Clicking Here