Purinergic Receptors
Cambridge Bioscience, through our collaboration with ChanTest, offers access to GPCR screening and profiling services to the following purinergic receptor targets:
P2Y1
Synonym: P2RY1
Validated Assay/Platform: Calcium
Description: The human P2Y family of metabotropic receptors include eight accepted members, including P2Y1,2,4,6,11,12,13,14. P2Y2,4,11 are activated mainly by nucleoside triphosphates whereas P2Y1,6,12,13 are activated by nucleoside diphosphates. P2Y1 couples preferentially to Gq/11 and is expressed in a variety of tissues including platelets, endothelia, smooth muscle, CNS, osteoblasts and B-islet cells
P2Y6
Synonym: P2RY6
Validated Assay/Platform: Calcium
Description: The human P2Y family of metabotropic receptors include eight accepted members, including P2Y1,2,4,6,11,12,13,14. P2Y2,4,11 are activated mainly by nucleoside triphosphates whereas P2Y1,6,12,13 are activated by nucleoside diphosphates. P2Y6 couples preferentially to Gq/11 and is expressed in a variety of tissues including endothelia, smooth muscle, heart, gall-bladder, retina, placenta, spleen
P2Y11
Synonym: P2RY11
Validated Assay/Platform: Calcium
Description: The human P2Y family of metabotropic receptors include eight accepted members, including P2Y1,2,4,6,11,12,13,14. P2Y2,4,11 are activated mainly by nucleoside triphosphates whereas P2Y1,6,12,13 are activated by nucleoside diphosphates. P2Y11 couples both to Gq/11 and to Gs and is expressed in a variety of tissues including epithelia, smooth muscle, granulocytes, dendritic cells and kidney.
P2Y12
Synonym: P2RY12, SP1999, HORK3, P2TAC, or P2T
Validated Assay/Platform: Calcium
Description: The human P2Y12 is an ADP receptor involved in platelet aggregation. ADP initiates platelet aggregation by simultaneous activation of two G protein-coupled receptors, P2Y1 and P2Y12. Activation of P2Y1 stimulates phospholipase C and triggers shape change, while P2Y12 coupled to Gi also involved in modulating thrombosis and hemostasis and therefore is a good drug target for antithrombotic drugs. P2Y receptors are expressed in a variety of tissues including platelets, endothelia, smooth muscle, CNS, osteoblasts , β-islet cells as well as in commonly used CHO-K1 and HEK293 cells. We therefore chose to express the P2Y12 receptor in 1321N1 astrocytoma cells, which have minimal endogenous receptor.
Platforms Available
Cambridge Bioscience, through our collaboration with ChanTest, offers access to both FLIPRTETRA® and FlexStation® 3 allowing you to select the right balance of speed, throughput and data quality to suit your needs.
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