Lysophospholipid Receptors
Cambridge Bioscience, through our collaboration with ChanTest, offers access to GPCR screening and profiling services to the following lysophospholipid receptor targets.
EDG1 (S1P1)
Synonym: S1P1
Validated Assay/Platform: Binding & cAMP
Description: Edg receptors constitute of family of 8 GPCRs (designated as Edg-1 through Edg-8) that can be further sub-classified as sphingosine 1-phosphate receptors (S-1-P) and lysophosphatidic acid (LPA) receptors. The Edg-1 receptor is an S-1-P receptor that is coupled to a variety of intracellular responses via Gi and Go. The Edg-1 receptor was initially cloned as an immediate-early gene induced during differentiation of human endothelial cells, an in vitro model of angiogenesis. It has been shown to play a critical role in regulating cellular proliferation and survival.
LPA1
Synonym: LPA1, EDG2
Validated Assay/Platform: Binding & cAMP
Description: Lysophosphatidic acid (LPA) is a small, bioactive phospholipid that mediates multiple cellular responses, including proliferation, differentiation, motility, and survival in both normal physiology and disease. The extracellular actions of LPA are mediated primarily through its interaction with five G protein-coupled receptors which include LPA1(EDG2), LPA2(EDG4), LPA3(EDG7), LPA4 (GPR23 or P2Y9) and LPA5 (GPR92). Among them, LPA1 (EDG2) is most widely expressed in normal tissue during growth and development. After LPA stimulation, LPA1 (EDG2) couples to Gi and G12/13 and was reported to play an important role in development of chronic neuroimmune disease and initiation of neuropathic pain.
LPA4
Synonym: GPR23 & p2y9
Validated Assay/Platform: Calcium
Description: LPA4 (GPR23) is lysophosphatidic acid receptor that is distantly related to the Edg family of receptors. It is expressed in significant abundance in ovary. When stimulated with LPA, this cell line showed a robust increase in intracellular calcium mobilization, whereas wild-type HEK293 cells show only a small response at very high concentrations of LPA.
Platforms Available
Cambridge Bioscience, through our collaboration with ChanTest, offers access to both FLIPRTETRA® and FlexStation® 3 allowing you to select the right balance of speed, throughput and data quality to suit your needs.
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