Loading Controls
Housekeeping proteins such as GAPDH, cytoskeletal actin and G6PD are useful as loading controls in western blot to normalise protein levels, to confirm gel to membrane transfer and check for edge effects. However house-keeping protein expression levels can vary between cell lines, different cellular compartments and treatment conditions meaning different loading controls are optimal in different systems.
Cambridge Bioscience offers a range of antibodies suitable for use as loading controls. All our antibodies are guaranteed to perform as stated in the datasheet and are covered by our Antibody Guarantee.





