Library Screening

Cambridge Bioscience provides access to a library screening service from Lucigen. This service aims to assist clients with identifying BAC and/or fosmid clones from custom BAC/fosmid libraries.

Library Screening Process
Library screening is performed via hybridization using synthetic oligonucleotide probes (one or more overgo probes) to custom-prepared nylon blots. Each blot is a 22 x 22cm nylon filter with 18,432 BAC/fosmid clones spotted in duplicate. The overgo probe is designed and generated from 24-mers containing an 8-base pair overlap. The probe is made radioactive in a fill-in reaction containing 32P-dATP, 32P-dCTP (Mc Pherson et al. Nature. 2001, 409:934-941). The resulting probe is a high specific activity 40mer which is used for hybridization to the nylon filters. Positive clone(s) identified will be provided as glycerol stocks.

Data Provided
At the completion of the project researchers are provided with positive clones in glycerol stocks. A complete report of screening results is also provided.

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