Gridding & High Density Colony Filters
Cambridge Bioscience offers access to a gridding and high density colony filtering service from Lucigen to facilitate the screening of large numbers of DNA clones.
Gridding & High Density Colony Filtering Process
Viable, bacterial cells from 96- or 384-well plates are robotically printed onto high-density, 22.2 × 22.2 cm nylon filters. After overnight growth of the clones, the filters are processed. As a result, DNA from each clone is permanently fixed to a known position on the filter, allowing rapid screening using standard DNA hybridization procedures. Positive clones can then be identified and retrieved from the original plates. Each nylon filter can be screened 3-5 times.
Lucigen provides 22.2 cm × 22.2 cm or 8 × 12 cm filters printed at a choice of two densities. All clones are printed in duplicate.
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