Fosmid Library Construction
Cambridge Bioscience offers access to a fosmid library construction service from Lucigen. This service uses the exclusive Random Shear Technology and the high efficiency CopyRight® v2.0 pSMART FOS vector
Benefits & Features of Random Shear Technology & CopyRight® v2.0 pSMART FOS Vector
There are numerous benefits provided by the combination of the exclusive random shear technology and CopyRight® v2.0 pSMART FOS vector offered by Lucigen.
• Unbiased Fosmid Libraries
This combination of technology eliminates the bias in DNA preparation and cloning. Clone gaps and sequencing gaps are practically eliminated and the potential problem of generating chimeric clones is eliminated.
• Large Fosmid Inserts Available
Conventional fosmid libraries have a limitation of an average insert size of 40 kb. The average insert size provided with this service is up to 90 kb with a minimal (~10 kb) window of variation
• Exceptional Yield & Small DNA Input Required
Conventional fosmid cloning requires high concentration and microgram quantities of purified large-insert DNA for ligation and packaging. High efficiency CopyRight v2.0 FOS vectors and the E. cloni® FOS Replicator™ Cells substantially increase recombinant yields produced by this service enabling the use of 100-fold less DNA.
• Exceptional Success Rate
The CopyRight v2.0 system ensures high yields of Fosmid DNA, resulting in a >95% success rate in Fosmid end-sequencing
• Simple & Fast
This service enables the simple and quick production of fosmid libraries
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To discuss a fosmid library construction project, please contact our experienced team by Clicking Here