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High Efficiency Electroporation Kits
Genome-Wide CRISPR/Cas-9 Knock-Out Kits

CRISPR/Cas9 Genome Engineering

The recent discovery of the CRISPR/Cas9 complex has provided researchers with an invaluable tool to target and modify any genomic sequence with high levels of efficacy and specificity. The CRISPR/Cas9 system uses a nuclease, CRISPR-associated Cas9 that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adaptor motif (PAM) in any genomic location. The system is comprised of separate guide RNAs (crRNA and tracrRNA) combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA sequence linked to the tracrRNA.

Cambridge Bioscience offers a comprehensive portfolio of tools and custom services for CRISPR/Cas9-based genome engineering including vector-based, mRNA/gRNA-based and Cas9 protein/gRNA-based systems and custom services. The genome engineering custom services include a range of options for optimal gRNA, HR donor vector design, full custom cloning and cell line engineering or design services if you wish to do cloning yourself. The custom service provides support and expertise with detailed notes for gene knock-out, knock-in, editing, and tagging applications.

Featured products within CRISPR/Cas9 Genome Engineering